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Articles by Khaled Hibar
Total Records ( 4 ) for Khaled Hibar
  Khaled Hibar , Mejda Daami-Remadi , Fakher Ayed and Mohamed El Mahjoub
  Fusarium oxysporum f. sp. radicis-lycopersici (FORL) is a new emerging pathogen in Tunisia. It causes Fusarium crown and root rot (FCRR) of tomato. Being a new disease, no control methods are available. Therefore, looking for a solution to this pathogen is required. In this study, the efficacy of some chemical fungicides to suppress FORL was evaluated in vitro, in growth chamber as well as under greenhouse conditions. In in vitro tests, all fungicides inhibited mycelial growth of FORL at 75 to 90% with the exception of maneb which entailed the lowest growth inhibition estimated at 40%. Under growth chamber trials, with the exception of benomyl, the other tested fungicides (Hymexazol, Hydroxyquinolin, Sodium Tetraborohydrate Decahydrate, Oxyquinolin and Flutriafol+Thiabendazole) have significantly reduced disease incidence. Under greenhouse conditions, results were more encouraging. Indeed, the use of Hymexazol reduces the percentage of dead plants at 6.4%. This study demonstrated the efficacy of some chemical fungicides in controlling FORL especially when they were incorporated to the culture substrate.
  Khaled Hibar , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  In this study, the efficacy of grafting two tomato cultivars onto two rootstocks was examined in growth chamber and in greenhouse conditions. The rootstock cultivars Beaufort F1 and He-Man F1, already known and confirmed as resistant to Fusarium oxysporum f. sp. radicis-lycopersici, were evaluated during two crop seasons under greenhouse heated with geothermal water in South Tunisia. The cv. Durintha F1 showed the best plant growth, fruit yield and fruit quality when grafted onto Beaufort F1; while cv. Bochra F1 gave the best results when grafted onto He-Man F1. This study demonstrated that grafting tomato cultivars onto Fusarium resistant rootstocks is one of the best alternatives for controlling Fusarium crown and root rot of tomato.
  Khaled Hibar , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  Fusarium crown and root rot of tomato (Lycopersicon esculentum) caused by Fusarium oxysporum f.sp. radicis-lycopersici is a new damaging disease of greenhouse crops in Tunisia. This pathogen was detected in Southern Tunisia, where temperature is usually high. Thus, temperature effect on its development was studied in vitro, on mycelial growth and in vivo, on disease incidence. Temperature effect on mycelial growth of Fusarium oxysporum. sp. radicis-lycopersici, evaluated on Potato Dextrose Agar (PDA) media, revealed that this pathogen grows well at temperatures ranged from 20 to 30°C. However, the optimum of mycelial growth was recorded at 25°C (growth speed exceeded 13 mm D-1). On disease incidence, temperature effect was evaluated by breeding inoculated plants under two thermal conditions: 19 and 29°C. Damages observed, after an incubation period ranged between 30 and 50 days, were more important at 19°C than that at 29°C. Indeed, at 19°C, disease incidence exceeded 96% and it was always more than 70%. At 29°C, however, this value didn`t exceed 60% and this by using two inoculation methods.
  Hayfa Jabnoun- Khiareddine , Mejda Daami- Remadi , Khaled Hibar , Jane Robb and Mohamed El Mahjoub
  Verticillium dahliae and Verticillium albo-atrum have been isolated from tomato cultivars possessing the Ve gene in many greenhouses in the Chott-Mariem region. Two isolates of Verticillium, one from each species, were examined for their mycelial growth at different incubation temperatures and their pathogenicity to three tomato cultivars. Seedlings at the three leaf stage were root-dipped in a conidial suspension (107 conidia mL-1) and maintained in two glasshouse cells at 17-21°C and 21-30°C. Disease severity was assessed through the index of leaf damage calculated 60 days after inoculation, at each temperature range. Growth test showed that V. albo-trum isolate as well as V. dahliae, were able to grow from 10 to 30 C. The three tomato cultivars exhibited typical Verticillium symptoms. The index of leaf damage differs significantly depending on cultivars, isolates and temperature ranges. V. albo-atrum was most virulent at 17-21°C; however, V. dahliae was more virulent at 21-30°C, despite the presence of the Ve-gene. These results confirmed the first report in Tunisia of a new pathotype of V. albo-atrum able to grow at 30°C and to infect cultivars having the Ve gene.
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