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Articles by L. Rajendran
Total Records ( 3 ) for L. Rajendran
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  In this study, for the management of Basal Stem Rot (BSR) disease sixty endophytic, rhizosphere strains were isolated from coconut, other crops and virgin soils. The strains showed high growth promotion were subjected to Ganoderma mycelium inhibition study in vitro. The strains EPC5 and EPC8 were showed high growth promotion and strong inhibition to Ganoderma pathogen compared to other strains. Both the strains were characterized by biochemical methods and confirmed as Bacillus. The Bacillus ITS region was amplified by specific primers and EPC5, EPC8 showed amplification of 546 bp products in size. Further, the strains were cloned and sequenced, deposited in NCBI, USA. The sequence showed similarity with Bacillus subtilis.
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  L. Rajendran and R. Samiyappan
  Endophytic bacterial strains were evaluated for their efficacy against the damping off disease caused by Rhizoctonia solani in cotton. Among hundred and three endophytic bacterial strains isolated, two strains (Bacillus sp. strains EPCO102 and EPCO16) significantly increased plant growth and inhibited the mycelial growth of Rhizoctonia solani in vitro conditions. The efficacy of talc-based bioformulation of endophytic bacterial strains, EPCO102, EPCO16 and Pseudomonas fluorescens strain Pf1 amended with and without chitin in inducing systemic resistance was tested against damping off disease under greenhouse conditions. The application of the bioformulation through seed, soil and foliar spray significantly reduced disease incidence under greenhouse conditions. The amendment of chitin in the formulation further reduced the disease incidence. EPCO102, EPCO16 and Pf1 strain along with chitin treatment was recorded 46.7, 53.3 and 40.0% damping-off incidence compared to control 83.3%. Treatment with the endophytic bacterial bioformulation increased the levels of the defence-related enzymes chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and phenol in cotton plants which had been challenged with R. solani. In addition to plant growth and antibiosis, endophytic bacterial strains enhanced the resistance in plants through the induction of defense enzymes in cotton plants.
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