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Articles by Luoyun Fang
Total Records ( 2 ) for Luoyun Fang
  Luoyun Fang , Hongfu Zhang , Feng Zhao , Junjun Wang and Defa Li
  The objective of this study was to determine enzyme activity in the digesta from the small intestine of growing pigs and develope an in vitro evaluation system for feed bioavailability using artificial small intestinal juice. Ten pigs (22.77±0.89 kg and fitted with a simple T-cannula at the jejunum) were used in a doubly 5x5 Latin square design. In each period, pigs were offered one of five diets differing in nutrient level for 14 days. The Standard diet (ST) contained 3400 kcal kg-1 of Digestible Energy value (DE) and 17% Crude Protein (CP). The DE for the other four diets was 3600, 3200, 3600 and 3200 kcal kg-1, respectively while the CP level was 21, 13, 13 and 21%, respectively. The small intestinal digesta was collected for determining digestive enzyme activity. Meanwhile, feces were collected for determining in vivo feed digestibility. The results showed that the range of amylase, trypsin, chymotrypsin and lipase activities in the intestinal fluid of growing pigs was 15.52-251.43, 21.24-67.39, 3.45-19.17 and 0.02-3.59 U mL-1, respectively. To establish an in vitro evaluation system for feed bioavailability, Artificial Small Intestinal Juice (ASIJ) was prepared with mixed enzyme reagents based on the mean activities of amylase, trypsin, chymotrypsin and lipase in the digesta used to evaluate the five diets by three-stage enzymatic incubation. By comparing the DE of the five feedstuffs from the in vivo method and the digestibility of the feedstuffs from the in vitro ASIJ analysis, mathematical models for predicting in vivo DE of In Vitro Dry Matter (IVDM), Organic Matter (IVOM) and DE (IVDE) was established (DE = 0.1076xIVDM+0.3741, R2 = 0.34; DE = 0.1276xIVOM+1.6486, R2 = 0.31; DE = 0.4625xIVDE+7.2065, R2 = 0.71). There were no significant differences between the in vitro evaluation results and the developed in vitro method. Therefore, the system in this study based on ASIJ is a convinent and reasonably accurate method for in vitro evaluation of feed bioavailability.
  Luoyun Fang , Tingting Mao , Lingna Xie and Linshu Jiang
  The objective of this study was to determine if the supply of digestible energy, dry matter and organic matter in a mixture feedstuffs is equal to the total of the supply based on the digestible supply determined in the single feed ingredients. Nine feedstuffs were provided to formulate the three diets which consisted of corn, soybean and wheat bran from the same region, the diets were formulated according to the requirements of growing pigs recommended by NRC. The dry matter and organic matter digestibility and digestible energy of nine feedstuffs and three diets were determined by in vitro method which simulates gastric, small intestine and large intestine digestion, the estimated digestible energy value of diets were calculated with predicted equation described by the previous experiment, the calculated digestible energy value of diets were calculated based on digestible energy value in single ingredients. Results showed that there were no differences among direct determined digestible energy, estimated digestible energy and calculated digestible energy. The directly determined dry matter, organic matter digestibility in diets were compared with those calculated digestibility based on determination in single ingredients, result showed that there were no differences between directly determined and calculated dry matter and organic matter digestibility in diet mixtures. In conclusion, the supply of digestible energy, dry matter and organic matter in a mixture of feedstuffs is equal to the total of the supply based on the digestible supply determined in the corn, soybean and wheat bran by in vitro method.
 
 
 
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