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Articles by M. Daami-Remadi
Total Records ( 3 ) for M. Daami-Remadi
  M. Daami-Remadi , K. Hibar , H. Jabnoun-Khiareddine , F. Ayed and M. El Mahjoub
  Trichoderma harzianum and T. viride were evaluated for their antagonistic activity against F. oxysporum f.sp. tuberosi, F. solani, F. graminearum and F. sambucinum causing potato dry rot in Tunisia. In vitro dual culture experiments, observed after incubation at 25°C for 6 days, showed that both tested Trichoderma species significantly reduced mycelial growth of Fusarium spp. comparatively to untreated controls and that a significant interaction was noted between both fixed factors (at p≤0.05). Light microscopic studies of antagonists x Fusarium spp. in vitro interactions showed lesser mycelium density, severe lysis, lesser pathogen sporulation, mycelial cords formation and early chlamydospores induction which were observed only at the confrontation zone of both microorganisms. Potato tubers, cv. Spunta, individually treated, at inoculation sites by 100 μL of Trichoderma spp. suspensions (108 spores mL-1), 24 h prior inoculation by Fusarium species, showed reduction in dry rot development, after 21 days of incubation at 25-27°C, comparatively to untreated controls.
  F. Ayed , M. Daami-Remadi , H. Jabnoun-Khiareddine and M. El Mahjoub
  Behaviour of some potato cultivars to Fusarium wilt caused by Fusarium oxysporum f.sp. tuberosi was studied. Plants were inoculated after two weeks by irrigation with a conidial suspension (107 spores mL-1). Variations of Fusarium wilt incidence were observed on potato 14 cultivars. Cv. Baraka was the most tolerant, Lyra and Platina were the most susceptible and Asterix, Alaska, Safrane and Timate were found to be intermediate with various degrees of response. Pathogen transmission to progeny tubers was assessed by planting daughter tubers coming from inoculated and non inoculated plants. Differences in incidence of cultivar susceptibility were significant. Disease incidence of Latona and Timate was the most and the least important, respectively.
  M. Daami-Remadi , H. Jabnoun-Khiareddine , F. Ayed , K. Hibar , I.E.A Znaidi and M. El Mahjoub
  Eight thermo-resistant fungi isolated from compost extracts are tested, for the first time in Tunisia, for their antagonistic activity, in vitro by dual culture with Fusarium spp. on PDA and in vivo by wound treatment prior to tuber inoculation. In vitro experiments showed that all tested compost fungi significantly inhibited mycelial growth of F. graminearum, F. sambucinum, F. solani and F. oxysporum f.sp. tuberosi, observed after incubation at 25°C during 6 days, revealing a higher competition. Light microscopic studies of tested pathogen mycelium, removed from the confrontation zone of both microorganisms, showed multiple mechanisms of action including mycoparasitism, lysis, early formation of chlamydospores and induction of mycelial cords via anatomosis between mycelial filaments. Tuber (cv. Spunta) treatment, 24 h before their inoculation by individually thermoresistant fungi suspensions, reduced development of dry rot after incubation for 21 days at 25-27°C, comparatively to the untreated control and this for the majority of the Fusarium complex tested.
 
 
 
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