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Articles by M. Bandehpour
Total Records ( 5 ) for M. Bandehpour
  M.R. Roozbahani , M. Bandehpour , A. Haghighi- Khiabanian-Asl , H. Abdollahi and B. Kazemi
  The aim of this study was designing a diagnostic kit for yersiniosis in the trout fish in Iran. Colonies of Yersinia ruckeri were collected from culture medium and a suspension was prepared in a lysing solution. DNA was extracted through a boiling and phenol chloroform method. Two primer sets targeting bacterial 16S rRNA and trout 18S rRNA. Polymerase chain reaction products were separated by gel electrophoresis. Among 20 suspected samples tested two samples were positive for both host and bacterial PCRs indicating the positive Y. ruckeri infection and remaining 18 samples were negative for pathogen. The performance of PCR reactions in negative samples were confirmed from amplification of internal control reactions targeting host. A PCR based diagnostic kit with an internal control was prepared for detection of Yersinia ruckeri in rainbow trout fish.
  A. Haghighi , S. Rasti , E. Nazemalhosseini Mojarad , B. Kazemi , M. Bandehpour , Z. Nochi , H. Hooshyar and M. Rezaian
  The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. Serine-rich protein is also present and polymorphic in Entamoeba dispar which called SREDP. The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. The results demonstrate an extensive genetic variability among Iranian E. dispar isolates. The repeat-containing region of the SREDP was found extensively polymorphic in size, number and order of repeat units. Genetic diversity of Iranian E. dispar isolates based on the SREDP was more polymorphic in comparison of Serine-Rich Entamoeba histolytica Protein (SREHP) of the E. histolytica isolates as well as were different from a few known SREDP genes.
  A.M. Saberi , M. Bandehpour , M. Afsharnasab , E. Ghayour , S.A. Yousefi Namin and B. Kazemi
  The aim of this study is designing a diagnostic kit for white spot syndrome virus. We designed 2 series of primers for diagnosis of viral VP24 gene and also primers as internal controls which amplify part of genome in both positive and negative samples. DNA of shrimps were extracted and PCR amplification carried out. In this research, a diagnosis kit for white spot disease of shrimps designed and tested using 32 shrimp samples which were dubious to have this disease. White spot virus were found in 23 samples and the other 9 were negative. For extra confirmation, the PCR product was sequenced and deposited to GenBank. We designed a diagnosis kit for white spot disease of shrimps and tested successfully.
  A. Haghighi-Khiabanian Asl , M. Azizzadeh , M. Bandehpour , Z. Sharifnia and B. Kazemi
  In this research, we confirmed the positive SVC in three Indian carps spp. (1) Rohu (Labeo rohita), (2) Merigal (Cirrhinus merigala) and (3) Catla (Catla catla) with typical histopathologic signs and PCR sequencing. Nested-PCR used to amplify a fragment of viral glycoprotein gene in Shahid Beheshti University M.C. and PCR product was purified and submitted to sequencing and deposited to GenBank at accession No. FJ711168. Two sites in this research that, were placed in Khuzestan Province (Ahvaz City, South of Iran) and Gilan Province (Astaneh Ashrafieh City, North of Iran) aquaculture farms. Samples were prepared for PCR method in both sites (30 pieces from Khuzestan and 30 pieces from Gilan Province), that all of the (100%) samples were positive in Nested-PCR. In addition, other 60 samples for histopathologic studies in both sites (30 pieces from Khuzestan and 30 pieces from Gilan Province), (according to 10% prevalence). All of the (100%) typically samples were with major histopathologic signs. The results of this study indicate that SVC infection can be found in some Indian carp in Iran.
  P. Ghadam , S. Gharavi , F. Yarian , M.R. Soudi , B. Kazemi and M. Bandehpour
  Among some Bacillus species, a protein highly homologous to HU, classified HB and coded by hbs gene. According to the recent studies, the sequence of hbs gene just in one strain of Bacillus subtilis exists in gene bank (ATCC 23857). In this study, DNA from Bacillus subtilis ATCC 6633 was extracted and investigated by PCR. The PCR product was sequenced and shown to differ in just one nucleotide from B. subtilis ATCC 23857. Hence, it was chosen as reference and for the first time, used for non-radioactive labeled probe preparation. The PCR product in Bacillus subtilis with ATCC 6633 was labeled using non-radioactive DIG-labeled nucleotides and conditions of probe preparation and hybridization were optimized and checked it by Southern blotting.
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