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Articles by M. Karthik
Total Records ( 2 ) for M. Karthik
  R. Hemalatha , M. Karthik , K. Narendra Babu and B. Dinesh Kumar
  Triticum aestivum which refers to the young grass of the common wheat plant has become extremely popular in treating a variety of health conditions. In this study we have investigated the immunomodulatory activity of Triticum aestivum water extract in Swiss albino mice and its effect on Th1/Th2 cytokine production by spleen cells. Female Swiss albino mice were challenged with Sheep Red Blood Cells (SRBC) and treated with either Triticum aestivum 540 mg kg-1 or Prednisolone 5 mg kg-1 body weight for 15 days. Blood was collected from retroorbital plexus to perform hematological, serological and bone marrow cellularity evaluation. Th1/Th2 cytokines and the p65 subunit of the nuclear factor kappa B (NFκB) were estimated in the splenocytes by ELISA from supernatants of splenocyte culture. Antioxidant activity of Triticum aestivum was assessed by using DPPH (2,2'-diphenyl-1-picrylhydrazyl) Assay. Water extract of Triticum aestivum was found to increase White Blood Cells (WBC), Red Blood Cells (RBC) and Hemoglobin (Hb) concentration in both normal and myelosuppressed Swiss albino mice. Furthermore, there was significant increase in bone marrow cellularity and hemagglutinin (antibody to SRBC) titer in animals treated with Triticum aestivum compared to control group. Triticum aestivum water extract upregulated Th1 cytokines (TNF-α, IL-2 and IFN- γ) and Th2 cytokine (IL4). In contrast, IL-1β (a Th1 cytokine) and P65 subunit of NFkB were suppressed in groups treated with Triticum aestivum. Moreover, Triticum aestivum extract restored Prednisolone suppressed TNF-α and IL-2 cytokines. Triticum aestivum appears to have a significant role in immunity and our findings confirm its beneficial role in hemoglobin concentration. Furthermore, the results on Th response suggest a potential role for Triticum aestivum in Th1 modulation and thus it’s potential role, as a candidate drug for inflammatory disorders including cancer management should be explored.
  R. Hemalatha , K. Narendra Babu , M. Karthik , R. Ramesh , B. Dinesh Kumar and P. Uday Kumar
  Ocimum sanctum (O. sanctum) has gained special attention due to its biological properties, however, little is known about its immunomodulatory effects. The purpose of this study was to investigate effect of O. sanctum on inflammation and immune response and its effect on Th1/Th2 cytokine production by spleen cells of myelosuppressed mice model. Female Swiss albino mice were challenged with SRBC and then were grouped and treated with either O. sanctum methanolic extract 850 mg kg-1 or Prednisolone 5 mg kg-1 body weight for 15 days. Blood was collected on 16th day from retro orbital plexus to perform hematological and immunological tests. Bone marrow cellularity was determined and supernatants of splenocytes cultures were analyzed for Th1/Th2 cytokines by ELISA. Antioxidant activity of O. sanctum was evaluated by DPPH (2,2'-diphenyl-1-picrylhydrazyl) assay. Treatment with O. sanctum showed significant increase in bone marrow cellularity (p<0.01), total WBC count (p<0.01) and hemoglobin concentration (p<0.01). In addition, there was a significant increase in SRBC antibody titer (1:1024) compared to the control group (1:256). O. sanctum increased the production of TNF-α, IL-2, IFN- γ and IL-4 (p<0.05) significantly and decreased the production of IL-1β and NF-kB. The methanolic extracts of O. sanctum showed free radical scavenging activity at 140 μg mL-1 concentration as IC50. This study documented improved haemoglobin concentration with O. sanctum treatment. These results support the use of this herb for wound healing and infection. The results also suggest potential use of O. sanctum as adjuvant in cancer therapy, myelotoxicity and in nutritional anemia.
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