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Articles by M.A. Bari
Total Records ( 13 ) for M.A. Bari
  A.H. Kabir , M.A. Bari , A.K.M.N. Huda , M.A. Rezvy and I. Mahfuz
  The investigation was based on direct shoot formation from shoot tip explant, subsequent morphogenesis and rooting of the in vitro proliferated shoots and transplantation of regenerated plants under ex vitro condition. Through proper growth regulators, it was possible to differentiate multiple shoots from shoot tip of papaya (Shahi). In the present investigation, it was observed that shoot proliferation was best in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1 and BAP 1.0 mg L-1+NAA 0.5 mg L-1. Maximum number of shoot per culture (28.2) and length of the largest shoot (1.7 cm) was found on MS medium supplemented with BAP 1.0 mg L-1+KIN 0.5 mg L-1. Shoot proliferation efficiency was found the best in 11 weeks old explant in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1. Sucrose in 30 g L-1 concentration as carbon source was proved to be best for shoot proliferation (82%). Maximum number of shoot per culture (41.2) and maximum length of shoot were obtained in the aforesaid concentration of carbon source. For adventitious rooting of regenerated shoot, MS medium containing IBA 1.0 mg L-1+NAA 0.5 mg L-1 was found to be the best. In this growth regulator, treatment 92% of rooting was observed. Longest length of the root (7.9 ± 0.121 cm) was found on MS medium supplemented with IBA 1.0 mg L-1+IAA 0.5 mg L-1. After transplanting the 20 days old rooted shoots into garden soil, compost and sand (2:1:1), 80% of survivability after 5 weeks was achieved.
  M. Asaduzzaman , M.A. Bari , M. Rahman , M. Minami , K. Matsushima and K. Nemoto
  Agrobacterium mediated transformation has already been reported in rice but under the present investigation efforts has been made to establish the transformation protocol in Bangladeshi rice variety BRRI dhan-30. High percentage of callus induction at 97.3% was obtained when seeds of rice (Oryza sativa L.) cv. BRRI dhan-30 were cultured on modified N6 medium supplemented with 3% sucrose, 2.0 mg L-1 2, 4-D and 0.8% agar under dark condition. Maximum  88.28%  calli  induced  shoots  and  were  obtained from BRRI dhan-30 in the suitable regeneration medium was MS medium supplemented with 3% (w/v) sucrose, 2.0 mg L-1 BAP+0.05 mg L-1 NAA+3% sorbitol and 0.8% agar. Agrobacterium mediated gene transfer protocol for rice variety BRRI dhan-30 were performed using Agrobacterium tumefaciens strain LBA4404, which harbored the plasmid pCL3 containing genes for β-glucuronidase (GUS) and kanamycin resistance. It was found that kanamycin concentration up 50 μg mL-1 were effective for selection of transformants. Putatives transformants as  indicated  by  kanamycin  test  were  subjected  to GUS assay. GUS activities were found in rice calli after co-cultivation.
  A.K.M.N. Huda and M.A. Bari
  This study describes the investigation on the technology of synthetic seed production using somatic embryos in two varieties (Loda and China) of eggplant. To standardize a media composition of artificial endosperm of synthetic seed, different concentrations and combinations of phytohormones with MS media were used in seed bead to achieve optimum germination rate on MS0 media. Among the different concentrations and combinations, MS+1.0 mg L-1 BAP+0.1 mg L-1 GA3 gave the highest germination rate and it was 81% for Loda and 70% for China variety. The influence of storage at 4°C and 0°C temperature on germination rate was also examined. This investigation indicates that synthetic seed could be stored at 4°C temperature for 45 days.
  N.A. Siddique , Jaime A. Teixeira da Silva , M. Anisuzzaman and M.A. Bari
  A procedure for rapid in vitro propagation of the aromatic and medicinal plants Aristolochia indica Linn and Hemidesmus indicus R.B (Family: Aristolochiaceae and Asclepiadaceae respectively) from axillary shoots is described. The highest percentage of callus induction was 92% on MS medium supplemented with 1.0 mg L-1 α-naphthalene acetic acid (NAA) and 1.0 mg L-1 6-benzyladenine (BA) for A. indica while 98% was achieved on MS medium supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 Kinetin (Kn) for H. indicus. The colour of the calli was mostly light to dark green. Development of adventitious shoots occurred when calli were subcultured on MS medium supplemented with BA and Kn alone or in BA combined with NAA and IAA, or NAA, IAA and BAP combined with Kn. The highest percentage (90%) of shoot regeneration in A. indica was obtained on MS medium fortified with 1.0 mg L-1 BA and 2.5 mg L-1 NAA, but a 98% rate on MS medium supplemented with 2.5 mg L-1 Kn and 1.0 mg L-1 NAA for H. indicus. Regenerated shoots rooted best on MS medium containing 2.5 mg L-1 Kn and 2.0 mg L-1 IBA. Plantlets were transferred to pots containing sand and soil mixture and acclimatized in a culture room. Finally rooted plants were transferred to soil.
  K.K. Paul and M.A. Bari
  The effects of various growth regulators on organogenesis from cormel axillary bud meristems of cultivated species of Maulavi kachu (Xanthosoma sagittifolium (L). Schott. were investigated. Highest percentage (65%) direct regeneration takes place within 25 days of culture but significant number of shoot per plant and length of shoot did not found significantly. Highest percentage (90%) calluses were initiated from axillary buds on MS medium supplemented with 1 mg L-1 BAP+2.0 mg L-1 NAA. Light green, greenish friable or semi friable calluses were found. The highest fresh weight of callus was 11.60 g. These calluses were cultured into MS media containing with 3 mg L -1 Kn + 2 mg L-1 NAA to induce plantlets regeneration. Highest percentage (86%) regeneration was observed with single or aggregated green shoot buds. The average number of green shoot, daughter buds and plantlets were 2.60 and average length of shoots were 7.0 cm with the same medium. Highest percentage of root induction and proliferation were also found into media containing 0.4 mg L-1 IAA and number of roots and root length of regenerated plantlets were 7.90 No. and 6.90 cm, respectively. Plantlets were then established with normal and natural fertile soil on plastic pot showed best performance in acclimatization.
  M.J. Hossa in , M. Rahman and M.A. Bari
  The aim of this study to show, an efficient protocol for establishment of cell suspension culture and plantlet regeneration through cell culture from the cotyledonary explants of Brinjal (Solanum melongena L.). In this investigation, three varieties of Brinjal cv. Loda, China and Jhotika were used. In first step, the somatic embryogenic calli formation was done using MS medium supplemented with different concentrations of auxin and cytokinin singly or in combination. Cells of the three varieties were isolated from the rapidly growing embryogenic and friable calli using orbital shaker. For callus induction the isolated cells were transferred to MS liquid medium containing different hormonal concentrations and after 37-63 days of incubation the micro-calli were appeared. The Loda and China varieties showed the best result (8.0 and 8.2%, respectively) in 2 mg L-1 NAA+0.05 mg L-1 BAP and 2 mg L-1 2,4-D+0.05 mg L-1 BAP. For embryo formation, micro-calli were subcultured on MS solid medium and the Loda variety showed the best result (21%) in the medium containing 1.0 mg L-1 BAP+0.05 mg L-1 GA3. The bipolar embryos were selected and cultured in MS medium with different combinations and concentrations of auxin (NAA) and cytokinin (BAP and IBA) for shoot and root formation. Optimum shoot and root formations were recorded in MS medium supplemented with 0.75 mg L-1 NAA+1.5 mg L-1 BAP and 2.0 mg L-1 NAA+0.5 mg L-1 IBA, respectively. The plantlets appeared in the embryo mass were cultured and acclimatized.
  L.A. Banu and M.A. Bari
  The experiment was conducted to Plant Biotechnology Laboratory of Institute of Biological Sciences, Rajshahi, Bangladesh. Shoot tip and leaf explants of Ocimum sanctum Linn. were cultured on different concentrations and combinations of growth regulators (BAP, Kin, 2, 4-D, IAA and IBA) in MS medium to observe shoot multiplication, callus induction, callus regeneration and root induction. Among the different concentrations and combinations of growth regulators, the highest percentage of shoot formation and highest average number of shoots were observed 90 and 5.88%, respectively in 0.2 mg L-1 BAP from shoot tip explants. Callus induction was obtained within 12-15 days of culture from leaf explants. The highest frequency (90.00%) of organic callus induction was observed in MS medium containing 1.0 mg L-1 NAA. Shoot regeneration occurred when the calli were sub cultured in Ms medium supplemented in BAP formulation. The highest percentage of shoot regeneration was obtained 90.00 in 0.2 mg L-1 BAP. In vitro grown shoots rooted best on MS medium containing 0.1 mg L-1 NAA. The in vitro grown plant lets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  N.A. Siddique and M.A. Bari
  N.A. Siddique , M.H. Kabir and M.A. Bari
  N.A. Siddique , Jaime A. Teixeira da Silva and M.A. Bari
  A.K.M.N. Huda , M. Rahman and M.A. Bari
  This study describes the investigation on the synthetic seed technology in two varieties (Loda and China) of eggplant with a particular focus on the carbon source in seed bead. To assess exact type and concentration of carbon source, three types of carbon sources (viz., sucrose, mannitol and sorbitol) and their different concentrations were used in alginate seed bead to achieve the optimum germination of synthetic seed on MS0. Among the different carbon sources, sucrose showed better performance for both varieties when nodal segment with bud was used as explants. But when somatic embryos were used as explants sucrose + sorbitol (1:1) was found to be more efficient for both varieties in germination of synthetic seeds. On the other hand, among the different concentrations 1% sucrose gave good result for the germination of synthetic seed made of nodal segment. In case of somatic embryo 3% sucrose showed best performance for both varieties.
  Sharmin Shahnewaz , M.A. Bari , N.A. Siddique , N. Khatun , M.H. Rahman and M.E. Haque
  Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinin). The best callusing from cultured anthers obtained in 2 medium containing 2,4-D 2 mg l-1, NAA 2.5 mg l-1 and kin 0.5 mg l-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg l-1, NAA 1 mg l-1, BAP 1.0 ml l-1 and incubated at 25±1OC in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.
  A.K.M.N. Huda , M.A. Bari , M. Rahman and N. Nahar
  This research programme was undertaken with a view to develop an efficient and reliable method of indirect regeneration through somatic embryogenesis in two varieties of eggplant through the technique of tissue culture. For this purpose, cotyledon pairs of Solanum melongena were used as explants for callus induction. Three different auxins (NAA, 2, 4-D and IAA) were used singly or in combination with BAP for present investigation, as types, concentrations and combinations of growth regulators had marked influence on callus induction. Auxin/cytokinin combinations were proved more potent for callus induction as well as callus growth than that of auxin tried singly in the media. Best callus formation was observed in MS media when supplemented with 0.05 mg L-1 BAP + 2.0 mg L-1 NAA. Best calli from cotyledonary explant were subcultured on MS supplemented with different combinations and concentrations of phytohormones for somatic embryogenesis. Among the different combinations and concentrations 0.05 mg L-1 BAP + 2.0 mg L-1 NAA gave the best result on somatic embryogenesis for Loda variety. On the other hand, somatic embryos of China variety were obtained only on the media having 1.0 mg L-1 BAP + 0.05 mg L-1 GA3. The global structure somatic embryos originated from the superficial layers of the calli, as a single structure or in a cluster. These structures could be isolated easily from parent tissues because they were floated freely in water. These floated free embryos were cultured on nutrient media again to obserb shoot primordia development and thereafter shoot development.
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