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Articles by Muhammad Akbar Shahid
Total Records ( 3 ) for Muhammad Akbar Shahid
  Muhammad Akbar Shahid , Muhammad Siddique , Sajjad-Ur-Rehman , Sajid Hameed and Abid Hussain
  The present study was conducted to monitor the presence of antibiotic residues in poultry meat. Swab Test on Animal Food (STAF) employing Bacillus subtilis as test organism on nutrient agar with 0.4% dextrose at pH 7, was used. A spore suspension of 2x107 spores mL-1 was used in 100 mL of nutrient agar to make STAF plates. A total of 100 tissue samples (33 liver, 33 kidney and 34 muscles) were collected from local market of Rawalpindi and Islamabad. Thirteen (39.4%) liver, 9 (27.3%) kidney and 7 (20.6%) muscle samples were detected positive for antibiotic residues. Antibiotic sensitivity and Minimum Inhibitory Concentration (MIC) of Oxytetracycline, Gentamicin, Neomycin, Enrofloxacin, Chloramphenicol, Amoxicillin, Benzyl Penicillin, Streptomycin and Tylosin against Bacillus subtilis was determined to be 0.6100, 0.0549, 0.1525, 0.1525, 0.0023, 0.0143, 0.0095, 0.0380 and 0.0023 μg, respectively. It is concluded from the study that STAF test can be used for the preliminary detection of antimicrobial residues in poultry tissues.
  Muhammad Akbar Shahid , Muhammad Siddique , Muhammad Abubakar , Muhammad Javed Arshed , Muhammad Asif and Arfan Ahmad
  In present study, status of Oxytetracycline (OTC) residues in chicken meat was determined in Rawalpindi/Islamabad area of Pakistan. Preliminary screening of samples for the presence of antibiotic residues was performed by a microbiological assay using Bacillus subtilis as test organism. OTC in positive samples was detected and quantified using High Performance Liquid Chromatography (HPLC) following extraction of the analytes with McIlvaine buffer and Solid Phase Extraction (SPE) system. A linear calibration curve was obtained with correlation coefficient of 0.9981 while average recoveries were greater than 91% with RSD values between 1.64 to 2.07%, while the Limit of Detection (LOD) was 0.01 μg mL-1. Out of 29 meat samples that were analyzed for OTC residues, 13 (44.8%) had detectable residue levels for OTC and 6 (20.7%) had higher residue levels than the recommended maximum residue level (0.2, 0.6 and 1.2 μg g-1) for muscles, liver and kidney, respectively. The method described in this study would be useful for routine monitoring of OTC residues in chicken meat.
  Abid Hussain , Abdul Shakoor , Muhammad Akbar Shahid , Muhammad Numan and Fahad Gulraiz
  Mastitis continues to be a major economic issue for dairy producers all over the world. Staphylococcus aureus is frequently isolated from bubaline mastitis and brings about glandular tissues changes obliterating the milk producing cells. Elimination of this organism from dairy herds requires treatment of infected mammary glands with antimicrobial agents and aggressive culling of refractory animals. The present study was designed to evaluate the disease characteristics of Staphylococcus aureus mastitis in buffaloes and antibiotic susceptibility profiles of isolates. Visual inspection and palpation indicated as; asymmetry between hind quarters (n = 3), symmetry between hind quarters (n = 3), asymmetry between front quarters (n = 1), symmetry between front quarters (n = 5), Clinical symptoms (n = 2), udder and teat’s wounds (n = 2), scar tissue (n = 1), warts (teat) (n = 1) and all others parameters were normal. A total of 100 milk samples (20 clinical and 80 sub-clinical) from mastitic quarters of buffaloes were collected. The antibiotic susceptibility profiles of recovered isolates Staphylococcus aureus were as; co-trimaxazole (100%), oxytetracycline (95.65%), amoxicillin (86.95%), gentamycin (86.95%), ampicillin (82.60%), ciprofloxacin (82.60%), chloramphenicol (82.60%), enrofloxacin (69.56%) and novabiocin (60.86%).
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