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Articles by Neji Tarchoun
Total Records ( 5 ) for Neji Tarchoun
  Amel Kerkeni , Mejda Daami-Remadi , Neji Tarchoun and Mohamed Ben Khedher
  Seven fungi isolated from an animal manure compost are tested for their in vitro and in vivo antagonistic activity against Pythium ultimum, the causal agent of the cucumber damping-off. In vitro, dual culture experiments, observed after incubation at 25°C on PDA during 4 days, showed that six fungi inhibited by 18 to 61% the mycelial growth of P. ultimum, whereas one fungus showed no activity. Competition for media was the most remarkable mechanism of action noted on PDA. In vivo experiments, sterilized peat individually treated with Aspergillus sp., Trichoderma viride (strain 1 and strain 2), at a rate of 0.5 g L-1 and infested with P. ultimum was evaluated for its suppressive effect of the cucumber (Cucumis sativus L. Mornegui) damping-off. Results showed the tested fungi, incorporated to the culture substrates, decreased the in vivo development of P. ultimum, but at variable degrees, comparatively to the untreated control. Damping-off was lower for cucumber plants treated with T. viride’ (strain 2) than those treated with Aspergillus sp. and with T. viride (strain 1). Aspergillus sp. isolated from compost, which was inactive in vitro, suppressed the cucumber damping-off by 69.4%.
  Amel Kerkeni , Mejda Daami-Remadi , Neji Tarchoun and Mohamed Ben Khedher
  Dual culture of some bacterial isolates obtained from animal manure compost extracts with F. oxysporum f. sp. radicis-lycopersici, the causal agent of the Fusarium Crown and Root Rot of tomato, significantly inhibited the in vitro development of the pathogen comparatively to the untreated control. Among 14 isolates tested, 8 inhibited by 38 to 47% the mycelial growth of F. oxysporum f. sp. radicis-lycopersici. The transplantation of tomato seedlings (cv. Riogrande) in peat, previously treated by bacterial suspensions and inoculated with a conidial suspension of the pathogen (107 spores mL-1), significantly reduced the Fusarium Crown and Root Rot severity compared to the untreated control. The most effective isolates were identified by means of the API system, as Chryseomonas luteola, Serratia liquifaciens and Aeromonas hydrophila.
  Amel Kerkeni , Mejda Daami-Remadi , Neji Tarchoun and Mohamed Ben Khedher
  .
  Amel Kerkeni , Mejda Daami-Remadi , Neji Tarchoun and Mohamed Ben Khedher
  In vitro experiments by direct confrontation, were conducted to assess the inhibitory effect of nine compost extracts, made with different mixtures of animal manure proportions, on some phytopathogenic fungi (Fusarium oxysporum f. sp. radicis-lycopersici, F. solani, F. graminearum, Fusicoccum amygdalis, Alternaria sp., Colletotrichum coccodes, Botrytis cinerea, Sclerotinia sclerotiorum, Aspergillus niger, Rhizoctonia solani, R. bataticola, Pythium sp. and Verticillium dahliae). Compost extracts decreased the radial growth of all fungi tested, except for Aspergillus niger. In fact, fungal radial growth inhibition ranged from 0 for A. niger to 63.17% for V. dahliae. Higher antifungal activity was also noted against F. oxysporum f. sp. radicis-lycopersici and F. solani with 38.12 and 31.87%, respectively. However, lesser inhibition, of about 10.33%, was obtained against R. bataticola. Compost extract C2, based on 60% cattle manure, 30% sheep manure and 10% ground straw and compost extract C7, based on 40% cattle manure, 40% sheep manure and 20% vegetable wastes, were found to be the most effective against the fungi tested. These extracts contain an important microflora which seem to be involved in this antifungal activity.
  Amel Kerkeni , Mejda Daami-Remadi , Neji Tarchoun and Mohamed Ben Khedher
  Aspergillus sp., Trichoderma viride strain 2 and T. viride strain 1 isolated from an animal manure compost are tested for their in vitro and in vivo antagonistic activity against Fusarium oxysporum f. sp. radicis-lycopersici, the causal agent of the Fusarium Crown and Root Rot of tomato. Dual culture experiments, observed after incubation at 25°C on PDA during 5 days, showed that all tested fungi significantly inhibited the mycelial growth of F. oxysporum f. sp. radicis-lycopersici comparatively to the untreated control. Inhibition varied from 25% for Trichoderma viride (strain 1) to 100% for Aspergillus sp. Competition for media was the predominant mechanism of action noted on PDA. In vivo, tomato plants (cv. Riogrande), simultaneously inoculated and treated individually by the compost fungi conidial suspensions (107 spores mL-1), showed reduced severity of the Fusarium Crown and Root Rot, when observed 30 days after transplantation, comparatively to the untreated control. The compost fungi T. viride (strain 1) was the most effective, it decreased severity of the disease by 48%.
 
 
 
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