Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
Temu kunci (Kaempferia pandurata (Roxb.)) has a number
of benefits and one of these is antibacterial. The rhizome is said to have antibacterial
activity against Streptococcus mutans, Lactocillus sp. and Candida
albicans. The aim of the study is to test the antibacterial activity of
Kaempferia pandurata (Roxb.) rhizome ethanol extract on methicillin-resistant
Staphylococcus aureus (MRSA), methicillin-resistant coagulase negative
Staphylococci (MRCNS), methicillin-sensitive Staphylococcus aureus (MSSA),
Bacillus subtilis and Salmonella typhi. Antimicrobial activity of the
extract was assayed by the microdilution method using Mueller Hinton Broth with
sterilized 96 round-bottomed microwells to determine the Minimum Inhibitory
Concentration (MIC) as well as to determine the time-kill activity. The MIC
of the extract was 16 ppm for both Bacillus subtilis and MRSA; 8 ppm
for both MSSA and Salmonella typhi and 4 ppm for MRCNS. Ethanol extract
of Kaempferia pandurata (Roxb.) showed antibacterial activity against
all the tested bacteria and was the most potent against MRCNS, with MIC
4 ppm. The killing profile test of the extract displayed bactericidal activity
at 8-16 ppm against MRSA, MSSA, Bacillus subtilis and Salmonella typhi
and bacteriostatic activity at 4 ppm towards MRCNS.
