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Articles by Ramaiyulis
Total Records ( 2 ) for Ramaiyulis
  Ramaiyulis , Madiati Zain , Rusmana Wijaya Setia Ningrat and Lili Warly
  Background and Objective: Protection of proteins is essential for productive ruminants, this study aimed to obtain optimal level of gambier leaf residue (GLR) that can protect the protein of cattle feed supplement (CFS) from rumen microbial degradation. Materials and Methods: Gambier (Uncaria gambir Roxb.) leaf residue containing 9.96% condensed tannin was added to CFS containing 29% crude protein with a mixture of brown sugar, soybean meal, coconut cake, tapioca, urea and minerals at level 0 (control), 2.5, 5.0 and 7.5%. Condensed tannin content in CFS: 0, 0.68, 1.17 and 1.60% then which were tested in rumen digestion in vitro. Results: The addition GLR in CFS can protected protein in CFS with decreased ruminal protein degradation 16.84% compare control. Rumen undegraded dietary protein (RUDP), TCA-soluble N and peptide N was increase by addition GLR in average 42.38% compared control. The optimum level of GLR in CFS was obtained 4.88% with the minimum rate of protein degradation in 1.05% h–1. Conclusion: The addition of GLR in CFS can protected protein from microbial degradation in rumen with optimum level of 4.88%.
  Ramaiyulis , Rusmana Wijaya Setia Ningrat , Mardiati Zain and Lili Warly
  Background and Objective: This study aimed to achieve optimal microbial protein synthesis by adding various levels of gambier leaf residue (GLR) to cattle feed supplement (CFS). Materials and Methods: Gambier (Uncaria gambir Roxb) leaf residue containing 9.96% condensed tannins was added at levels of 0, 2.5, 5.0 and 7.5% (DM) to CFS formula containing 29% crude protein and then tested in vitro rumen digestion. Results: Addition of 5% GLR significantly decreased the rumen protozoan population by 24.43% (p<0.05) and decreased the NH3-N content (p<0.01) but did not affect the pH or total volatile fatty acid (VFA) content (p>0.05). The mean of the microbial biomass was 111-285 mg 100–1 mL of rumen fluid and the microbial protein concentration was 59-157 mg 100–1 mL of rumen fluid, which was highest at 5.0 GLR (p<0.01). Overall, the rate of microbial biomass production, rate of microbial protein synthesis and efficiency of microbial protein synthesis were 7.30-10.18 mg 100–1 mL h–1, 3.86-5.62 mg 100–1 mL h–1 and 16-47 mg microbial protein 100–1 mg digested organic matter, respectively, with the highest rate at 5.0% GLR and the lowest rate in controls (p<0.01). Conclusion: Addition of 5.0% GLR to CFS can optimize microbial protein synthesis in the rumen.
 
 
 
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