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Articles by T. Raguchander
Total Records ( 3 ) for T. Raguchander
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  In this study, for the management of Basal Stem Rot (BSR) disease sixty endophytic, rhizosphere strains were isolated from coconut, other crops and virgin soils. The strains showed high growth promotion were subjected to Ganoderma mycelium inhibition study in vitro. The strains EPC5 and EPC8 were showed high growth promotion and strong inhibition to Ganoderma pathogen compared to other strains. Both the strains were characterized by biochemical methods and confirmed as Bacillus. The Bacillus ITS region was amplified by specific primers and EPC5, EPC8 showed amplification of 546 bp products in size. Further, the strains were cloned and sequenced, deposited in NCBI, USA. The sequence showed similarity with Bacillus subtilis.
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  R. Nandakumar , S. Babu , G. Amutha , T. Raguchander and R. Samiyappan
  Phytotoxic metabolite produced by the five different isolates of Sarocladium oryzae, the rice sheath rot pathogen, was isolated from its in vitro culture filtrate to know the variability among isolates in terms of toxin production. The toxin was purified through solvent extraction and then by gel filtration on sephadex column. Carbohydrate and protein content of the toxin and the quantity of phytotoxin produced in planta were varied among isolates. Toxin from the five isolates also showed variation in its biological activity as detected in its ability to induce sheath rot symptoms on detached leaf sheath, greenhouse grown plants and to cause leakage of electrolytes from rice leaf sheath. The symptoms produced by the purified toxin were, symptoms similar to those of natural infection by the pathogen. The virulent isolates SO1 and SO2 produced more amount of toxin, more leakage of electrolytes and severe sheath rot symptoms. The in planta toxin production by different isolates was detected serologically, using the polyclonal antibody produced against SO1 toxin. Correlation between the variation in toxin production and the virulence of the pathogen was discussed.
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