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Articles by T.O. Femi-Ola
Total Records ( 5 ) for T.O. Femi-Ola
  T.O. Femi-Ola and E.Y. Aderibigbe
  The effect of aqueous extracts of five wood samples: Khaya grandifoliola, Mansonia altissima, Brachystegia eurycoma, Milicia excelsa and Terminalia superba on some cellulolytic bacterial strains was investigated. The aqueous extracts of the wood samples inhibited the growth and cellulolytic activity of the Bacillus subtilis strains to varying degrees. The inhibitory effect of the extracts of Brachystegia eurycoma against the bacterial strains was highest when compared to the other wood types.
  T.O. Femi-Ola , V.A. Ajibade and A. Afolabi
  The proximate and mineral composition of raw and boiled African locust bean (Parkia biglobosa) was determined. The termicidal properties of the aqueous and acetone extracts of the beans were also investigated. There were variations in the proximate and chemical composition of the raw and boiled samples. Cadmium, nickel, lead, cobalt and copper were leached out of the seed during boiling. The aqueous and acetone extracts of boiled P. biglobosa had no effect on termites, while they were affected by extracts of raw P. biglobosa. The aqueous and acetone extracts exhibited concentration dependent termicidal activity. However the aqueous extract was more active than the acetone extract. Termites died within 40 and 110 min when exposed to paper pads treated with 0.4 g mL-1 of aqueous and acetone extracts, respectively.
  T.O. Femi-Ola and E.Y. Aderibigbe
  The bacterial population in the hindgut of the higher wood-eating termite, Amitermes evuncifer Silvestri was estimated at both dry and wet seasons. The total bacterial counts in the soldier and worker termites were 1.46±0.26x106 and 2.51±0.31x106 cfu mL-1 during the wet season; while it was 5.30±1.1x104 and 5.8±0.9x104 cfu mL-1 during the dry season, respectively. There was no significant difference in the total bacterial count in the hindgut of the worker and soldier termites in both seasons. The total bacterial count in the dry season was significantly lower (p≤0.05) than the population during the wet season. The bacterial species were identified to be Bacillus subtilis, B. cereus, Micrococcus luteus, Streptococcus sp. and Serratia marcescens.
  T.O. Femi-Ola , E.Y. Aderibigbe and L. Awoyemi
  The survival of Amitermes evuncifer (Silvestri) on five different Nigerian wood species: Terminalia superba Engl and Diels, Khaya grandifoliola C.D.C., Milicia excelsa Welw C.C. Berg, Mansonia altissima A. Chev and Brachystegia eurycoma Harms for seven days was investigated. Some physicochemical properties of the wood samples were also determined. The survival rate on Terminalia superba was highest (22.5%) and least on Mansonia altissima and Brachystegia eurycoma (0%), respectively. The other wood types had less than 15% live termites. Micrococcus luteus and Bacillus species were persistently isolated from the hindgut of the termites throughout the period of the no-choice food experiment. The total bacterial population in the hindgut of the termites declined from 104 to 102 cfu mL-1. The moisture content, density and pH of the wood samples ranged from 10 to 18%, 409 to 758 kg m-3 and 5.52 to 5.88, respectively. There was a high degree of correlation (R = 0.96; R2 = 0.922) between the amount of wood consumed and survival of A. evuncifer. However, the correlation coefficient between the amount of wood consumed and density of wood was negative (R = -0.675; R2 = 0.455).
  T.O. Femi-Ola and B.M. Olowe
  Amylase producer Bacillus subtilis BS5 was obtained from the hindgut of wood eating termites Amitermis evuncifer Silvestri. Amylase enzyme produced by this strain when grown on basal liquid medium with cocoyam starch as substrate was characterized. The enzyme was purified through ion-exchange chromatography and gel-filtration. The purified enzyme had a molecular weight of 63 kDa and was active optimally at pH 6.0 and 50°C. The apparent Km value and Vmax of the enzyme during hydrolysis of soluble starch were 16.67 mg mL-1 and 3.8 mg/min/mL-1, respectively. The activities of the amylase were stimulated by Ca2+, Na+ and Mg2+ but inhibited by EDTA and HgCl2.
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