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Articles by Neelam Verma
Total Records ( 3 ) for Neelam Verma
  Neelam Verma , Hardeep Kaur and Sachin Kumar
  Rapid industrialization, urbanization and modern agricultural practices have led to cumulative pollution of environment by heavy metals, thereby deteriorating water and food product quality. Milk and its products are most prone to heavy metal contamination and lead has been considered as a major contaminant in milk. A whole cell electrochemical lead biosensor has been developed using urease producing Bacillus sphaericus isolate (MTCC 5100) which was grown at 30°C for 24 h and then harvested in phosphate buffer saline. Urease enzyme units available in the biomass were 53.87±1.41 IU mL-1. Whole cells were immobilized at the tip of carbon paste electrode and unutilized NADPH oxidation has been studied to reveal the lead concentration in milk. Preconcentration and specificity has been achieved by lead specific column. Lead analysis has been done at 25°C in 5 min response time. Detection limit achieved is 2.4x10-3 nM well below the permissible limit of lead in milk. The developed method could be used for off line analysis of lead by portable handheld potentiostat.
  Atul Bhardwaj and Neelam Verma
  Background and Objective: Chlorpyrifos (CP) is one the most widely used broad spectrum organophosphorus (OP) pesticide in agricultural fields worldwide particularly in Punjab, India. Scarcity of the knowledge in farmers regarding the usage, handling and safety concerns of pesticides leads to massive and uninhibited use of CP. Upon hydrolysis, CP usually generates diethyl thiophosphoric acid (DETP) and 3,5,6-Trichloro-2-pyridinol (TCP), later one possess antimicrobial attributes. Residues of both CP and TCP have been detected in ecosystems and imparts numerous toxicological effects on the various life forms particularly in humans. Hence, their elimination from the contaminated environmental sites is highly needful and bioremediation is the most convenient option at present as it is cost-effective and ecofriendly. Thus objective of the present study was to isolate a bacterial strain having the capability to degrade CP and TCP both completely in to less toxic forms. Materials and Methods: Bacterial strain was isolated from CP and TCP contaminated soils of Malwa region of Punjab where CP has been used continuously in agricultural fields. The CP utilization capabilities of bacterial strain were analyzed by HPLC, HPTLC and other chemical based colorimetric methods. Minimum inhibitory concentration of CP and TCP on the strain was analyzed and also influence of pH and temperature on the growth of isolate was assessed. Results: Strain was found to be Gram positive and was able to use CP as well as TCP as sole carbon source. Molecular characterization based on 16S rRNA gene sequence homology confirmed its identity as Bacillus subtilis . The HPLC studies revealed almost complete degradation of 150 ppm of CP within 5 days without accumulation of TCP in the system. Isolate was competent to tolerate initial CP concentration as high as 800 ppm and present study report firstly of tolerating such higher concentration by Bacillus subtilis strain. Isolate was well adapted to grow within the wide temperature (25-40EC) and pH (6.0-9.0) range. Conclusion: The isolate Bacillus subtilis NJ11 was found to be efficient in the remediation of CP and TCP in liquid medium by converting them into lesser toxic intermediates. Present study suggested that strain has potential to clean up the CP and TCP contaminated sites.
  Varinder Kaur , Ashok Kumar Malik and Neelam Verma
  A new, simple, inexpensive, and sensitive method for the simultaneous spectrophotometric determination of Cu(II) and Pd(II) by H-point standard addition method and derivative spectrophotometry is described. 4- Phenylpiperazinecarbodithioate (PPDTC) in an anionic micellar solution of SLS was used as a reagent at pH 4. The experimental parameters, such as pH, type of surfactant, concentration of reagent, and surfactant used, were optimized to minimize the errors. The linear ranges of the method were 0.1-10 and 0.1-11 μg mL-1 for Cu(II) and Pd(II), respectively. The results of applying H-point standard addition and fourth derivative spectrophotometry showed that Cu(II) and Pd(II) can be determined simultaneously with detection limits of 0.12 and 0.11 μg mL-1 respectively. Therefore, the method of H-point standard addition and fourth derivative spectrophotometry was used for resolving overlapped spectra for the determination of Cu(II) and Pd(II). This method was successfully applied to the determination of these metals in synthetic and real samples.
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