Z.L. Zhou
PPPSRU/Agricultural Research Service/USDA, Fayetteville, AR 72701, USA
N.C. Rath
PPPSRU/Agricultural Research Service/USDA, Fayetteville, AR 72701, USA
G.R. Huff
PPPSRU/Agricultural Research Service/USDA, Fayetteville, AR 72701, USA
W.E. Huff
PPPSRU/Agricultural Research Service/USDA, Fayetteville, AR 72701, USA
K.S. Rasaputra
PPPSRU/Agricultural Research Service/USDA, Fayetteville, AR 72701, USA
C. Salas
Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA
C.N. Coon
Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA
ABSTRACT
The effect of two nutritional supplements, a Yeast Extract (YE) and a vitamin D3 formulation (VD) on growth and structural properties of bones from turkeys, transiently subjected to a simulated stress using Dexamethasone (Dex) administration, was determined. The birds were fed diets with or without YE or VD supplements during wks of 6, 11 and 15 post hatch. At weeks 6 and 15 of age, half of the birds in each treatment group received 3 intramuscular injections of Dex at concentrations of 2 mg/kg BW on 3 alternating days to induce simulated stress. At 16 wk of age, the birds were weighed, bled prior to euthanasia and the tibia were harvested at necropsy to determine their mineral content, density and biomechanical properties. Bone Mineral Density (BMD) and Bone Mineral Content (BMC) of whole tibia were determined by Dual Energy X ray Absorptiometry (DEXA) and the biomechanical properties using Instron material testing machine. The ash yield and bone densities were determined using bone marrow free mid diphyseal segments manually by ashing and Archimedes principle. Serum Ca, P, protein and alkaline phosphatase measured using a clinical chemistry analyzer. Neither YE nor VD had any effect on body or bone weights by itself or in combination. Dex reduced both BW and bone weights. DEXA estimated BMD and BMC of whole tibia were reduced in Dex-stressed birds but it was not evident measuring the diaphyseal bone and ash densities. Dex treatment lowered the breaking strength and the plasticity of bone but had no significant effect on its stiffness. Dex treated turkeys showed higher relative bone weights indicating faster recovery of bones from Dex induced growth suppression. Overall, these results suggest that decreased bone mass due to Dex-induced growth suppression reduces bone strength and can alter some structural properties. Intermittent treatment with either VD or YE individually or in combinations do not provide much protection against the negative effects of stress.
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How to cite this article
Z.L. Zhou, N.C. Rath, G.R. Huff, W.E. Huff, K.S. Rasaputra, C. Salas and C.N. Coon, 2011. Bone Characteristics of 16 Wk-Old-Turkeys Subjected to Different Dietary Supplements and Simulated Stress. International Journal of Poultry Science, 10: 332-337.
DOI: 10.3923/ijps.2011.332.337
URL: https://scialert.net/abstract/?doi=ijps.2011.332.337
DOI: 10.3923/ijps.2011.332.337
URL: https://scialert.net/abstract/?doi=ijps.2011.332.337
REFERENCES
- Bradshaw, R.H., R.D. Kirkden and D.M. Broom, 2002. A review of the aetiology and pathology of leg weakness in broilers in relation to welfare. Avian Poult. Biol. Rev., 13: 45-103.
CrossRefDirect Link - Dawkins, M.S., C.A. Donnelly and T.A. Jones, 2004. Chicken welfare is influenced more by housing conditions than by stocking density. Nature, 427: 342-344.
CrossRefDirect Link - Ferket, P.R., E.O. Oviedo-Rondon, P.L. Mente, D.V. Bohorquez and A.A. Santos Jr. et al., 2009. Organic trace minerals and 25-hydroxycholecalciferol affect performance characteristics, leg abnormalities and biomechanical properties of leg bones of Turkeys. Poult. Sci., 88: 118-131.
CrossRefDirect Link - Fritts, C.A. and P.W. Waldroup, 2003. Effect of source and level of vitamin D on live performance and bone development in growing broilers. Applied Poult. Res., 12: 45-52.
CrossRefDirect Link - Hester, P.Y., M.A. Schreiweis, J.I. Orban, H. Mazzuco, M.N. Kopka, M.C. Ledur and D.E. Moody, 2004. Assessing bone mineral density in vivo: Dual energy X-ray absorptiometry. Poult. Sci., 83: 215-221.
CrossRefDirect Link - Huff, G.R., W.E. Huff, J.M. Balog and N.C. Rath, 2000. The effect of vitamin D3 on resistance to stress-related infection in an experimental model of turkey osteomyelitis complex. Poult. Sci., 79: 672-679.
Direct Link - Huff, G.R., W.E. Huff, J.M. Balog, N.C. Rath, H. Xie and R.L. Horst, 2002. Effect of dietary supplementation with vitamin D metabolites in an experimental model of Turkey osteomyelitis complex. Poult. Sci., 81: 958-965.
Direct Link - Huff, G.R., W.E. Huff, N.C. Rath, F. Solis de los Santos, M.B. Farnell and A.M. Donoghue, 2007. Influence of hen age on the response of turkey poults to cold stress, Escherichia coli challenge and treatment with a yeast extract antibiotic alternative. Poult. Sci., 86: 636-642.
Direct Link - Julian, R.J., 2005. Production and growth related disorders and other metabolic diseases of poultry-A review. Vet. J., 169: 350-369.
CrossRefPubMedDirect Link - Knowles, T.G., S.C. Kestin, S.M. Haslam, S.N. Brown and L.E. Green et al., 2008. Leg disorders in broiler chickens: Prevalence, risk factors and prevention. PLoS ONE, Vol. 3.
CrossRefDirect Link - Lin, H., S.J. Sui, H.C. Jiao, J. Buyse and E. Decuypere, 2006. Impaired development of broiler chickens by stress mimicked by corticosterone exposure. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 143: 400-405.
CrossRefPubMedDirect Link - NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Direct Link - Patterson, J.A. and K.M. Burkholder, 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci., 82: 627-631.
CrossRefPubMedDirect Link - Rath, N.C., G.R. Huff, W.E. Huff and J.M. Balog, 2000. Factors regulating bone maturity and strength in poultry. Poult. Sci., 79: 1024-1032.
CrossRefPubMedDirect Link - Scholz-Ahrens, K.E., P. Ade, B. Marten, P. Weber and W. Timm et al., 2007. Prebiotics, probiotics and synbiotics affect mineral absorption, bone mineral content and bone structure. J. Nutr., 137: 838S-846S.
Direct Link - Schreiweis, M.A. J.I. Orban, M.C. Ledur, D.E. Moody and P.Y. Hester, 2005. Validation of dual-energy X-ray absorptiometry in live White Leghorns. Poult. Sci., 84: 91-99.
Direct Link - Talaty, P.N., M.N. Katanbaf and P.Y. Hester, 2010. Bone mineralization in male commercial broilers and its relationship to gait score. Poult. Sci., 89: 342-348.
CrossRefDirect Link - Turner, C.H. and D.B. Burr, 1993. Basic biomechanical measurements of bone. Bone, 14: 593-608.
Direct Link - Van Der Eerden, B.C., J.M. Karperien and J.M. Wit, 2003. Systemic and local regulation of the growth plate. Endocr. Rev., 24: 782-801.
CrossRefDirect Link - Virden, W.S., J.P. Thaxton, A. Corzo, W.A. Dozier and M.T. Kidd, 2007. Evaluation of models using corticosterone and adrenocorticotropin to in stress in commercial broilers. Poult. Sci., 86: 2485-2491.
CrossRefDirect Link - Watts, N.B., 2002. Bone quality: Getting closer to a definition. J. Bone Miner. Res., 17: 1148-1150.
PubMed - Westendorf, M.L. and J.E. Wohlt, 2002. Brewing by-products: Their use as animal feeds. Vet. Clin. Food Anim. Pract., 18: 233-252.
Direct Link - Whitehead, C.C., H.A. McCormack, L. McTeir and R.H. Fleming, 2004. High vitamin D3 requirements in broilers for bone quality and prevention of tibial dyschondroplasia and interactions with dietary calcium, available phosphorus and vitamin A. Br. Poult. Sci., 45: 425-436.
CrossRefDirect Link - Zioupos, P. and J.D. Currey, 1998. Changes in the stiffness, strength and toughness of human cortical bone with age. Bone, 22: 57-66.
Direct Link - Zotti, A., C. Rizzi, G. Chiericato and D. Bernardini, 2003. Accuracy and precision of dual-energy x-ray absorptiometry for ex vivo determination of mineral content in turkey poult bones. Vet. Radiol. Ultrasound., 44: 49-52.
PubMed