L. M. Stevenson
Department of Poultry Science, Auburn University, AL 36849, USA
J. B. Hess
Department of Poultry Science, Auburn University, AL 36849, USA
S. S. Oates
Department of Poultry Science, Auburn University, AL 36849, USA
W. D. Berry
Department of Poultry Science, Auburn University, AL 36849, USA
ABSTRACT
Abnormal liver lipid accumulation has a negative effect on health. It is associated with many factors including estrogen, alcohol consumption, metabolic problems and obesity. Research has shown that soy phytoestrogens may have a protective effect against liver lipid accumulation in rats. Exogenous estrogen has been used experimentally to induce liver lipid accumulation in chickens. A series of studies was performed to determine if genistein had a protective effect against liver lipid accumulation induced by exogenous estrogen. Three experiments were performed using different types and ages of chickens; old hens, young hens and male broiler chicks. Birds were randomly selected and divided into six treatment groups. All treatments doses were dissolved in dimethyl sulfoxide (DMSO) with sesame seed oil as a carrier. Genistein doses were given by a daily oral gavage for fourteen days. Estrogen doses were given by injection in the subcutaneous tissue in the back of the neck three times during the experiment. There were no significant differences in most of the items measured. There was a significant difference in the liver weight (p = 0.035) and relative liver weight (p = 0.01) for the study involving male broiler chicks. The amount of genistein in plasma and liver samples was measured by HPLC. There was a significant difference in the amount of genistein in the plasma of the old hens after treatment with genistein (p = 0.01). There was also a significant difference in the amount of genistein in the liver of the old hens (p = 0.003) and male broiler chicks (p = 0.005) after treatment with genistein.
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How to cite this article
L. M. Stevenson, J. B. Hess, S. S. Oates and W. D. Berry, 2014. A Comparison of the Effects of Estradiol and the Soy Phytoestrogen Genistein on Liver Lipid Content of Chickens. International Journal of Poultry Science, 13: 124-132.
DOI: 10.3923/ijps.2014.124.132
URL: https://scialert.net/abstract/?doi=ijps.2014.124.132
DOI: 10.3923/ijps.2014.124.132
URL: https://scialert.net/abstract/?doi=ijps.2014.124.132
REFERENCES
- Anthony, M.S., T.B. Clarkson and J.K. Williams, 1998. Effects of soy isoflavones on atherosclerosis: Potential mechanisms. Am. J. Clin. Nutr., 68 : 1390s-1393s.
PubMed - Ayala, I., A.M. Castillo, G. Adanez, A. Fernandez-Rufette, B.G. Perez and M.T. Castells, 2009. Hyperlipidemic chicken as a model of non-alcoholic steatohepatitis. Exp. Biol. Med., 234: 10-16.
CrossRefPubMedDirect Link - Bloedon, L.T., A.R. Jeffcoat, W. Lopaczynski, M.J. Schell and T.M. Black et al., 2002. Safety and pharmacokinetics of purified soy isoflavones: Single-dose administration to postmenopausal women. Am. J. Clin. Nutr., 76: 1126-1137.
PubMed - Chen, A.C. and S.M. Donovan, 2004. Genistein at a concentration present in soy infant formula inhibits Caco-2BBe cell proliferation by causing G2/M cell cycle arrest. J. Nutr., 134: 1303-1308.
PubMed - Dang, Z.C., V. Audinot, S.E. Papaoulos, J.A. Boutin and C.W. Lowik, 2003. Peroxisome proliferator-activated receptor gamma (PPARgamma ) as a molecular target for the soy phytoestrogen genistein. J. Biol. Chem., 278: 267-269.
PubMed - Gu, L., S.E. House, R.L. Prior, N. Fang, M.J. Ronis, T.B. Clarkson, M.E. Wilson and T.M. Badger, 2006. Metabolic phenotype of isoflavones differ among female rats, pigs, monkeys and women. J. Nutr., 136: 1215-1221.
PubMed - Heim, M., O. Frank, G. Kampmann, N. Schocky and T. Pennimpede et al., 2004. The phytoestrogens genistein enhances osteogenesis and represses adipogenic differentiation of human primary bone marrow stromal cells. Endocrinology, 145: 848-859.
PubMed - Dang, Z.C. and C. Lowik, 2005. Dose-dependent effects of phytoestrogens on bone. Trends Endocrinol. Metab., 16: 207-213.
CrossRefDirect Link - Hemre, G.I., M. Sanden, A.M. Bakke-McKellep, A. Sagstad and A. Krogdahl, 2005. Growth, feed utilization and health of Atlantic salmon Salmo salar L. fed genetically modified compared to non-modified commercial hybrid soybeans. Aquaculture Nutr., 11: 157-167.
CrossRefDirect Link - Hermier, D., P. Forgez, P.M. Laplaud and M.J. Chapman, 1988. Density distribution and physicochemical properties of plasma lipoproteins and apolipoproteins in the goose, Anser anser, a potential model of liver steatosis. J. Lipid Res., 29: 893-907.
PubMed - Hermier, D., A. Saadoun, M.R. Salichon, N. Sellier and D. Rousselot-Pailley, 1991. Plasma lipoproteins and liver lipids in two breeds of geese with different susceptibility to hepatic steatosis: Changes induced by development and force-feeding. Lipids, 26: 331-339.
PubMedDirect Link - Hermier, D., G. Guy, S. Guillaumin, S. Davail, J.M. Andre and R. Hoo-Paris, 2003. Differential channelling of liver lipids in relation to susceptibility to hepatic steatosis in two species of ducks. Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 135: 663-675.
CrossRefDirect Link - Ji, G., Q. Yang, J. Hao, L. Guo, X. Chen, J. Hu, L. Leng and Z. Jiang, 2011. Anti-inflammatory effect of genistein on non-alcoholic steatohepatitis rats induced by high fat diet and its potential mechanisms. Int. Immunopharmacol., 11: 762-768.
CrossRefDirect Link - Kim, S., I. Sohn, Y.S. Lee and Y.S. Lee, 2005. Hepatic gene expression profiles are altered by genistein supplementation in mice with diet-induced obesity. J. Nutr., 135: 33-41.
PubMedDirect Link - Kudzma, D.J., P.M. Hegstad and R.E. Stoll, 1973. The chick as a laboratory model for the study of estrogen-induced hyperlipidemia. Metabolism, 22: 423-434.
CrossRefPubMedDirect Link - Lee, Y.M., J.S. Choi, M.H. Kim, M.H. Jung, Y.S. Lee and J. Song, 2006. Effects of dietary genistein on hepatic lipid metabolism and mitochondrial function in mice fed high-fat diets. Nutrition, 22: 956-964.
CrossRefPubMedDirect Link - Luskey, K.L., M.S. Brown and J.L. Goldstein, 1974. Stimulation of the synthesis of very low density lipoproteins in rooster liver by estradiol. J. Biol. Chem., 249: 5939-5947.
PubMedDirect Link - McClain, R.M., E. Wolz, A. Davidovich, F. Pfannkuch, J.A. Edwards and J. Bausch, 2006. Acute, subchronic and chronic safety studies with genistein in rats. Food Chem. Toxicol., 44: 56-80.
CrossRefPubMedDirect Link - Manning, R., S. Courtney and S. Talbot, 1989. Temporal changes in plasma and liver lipids and in the hepatic activities of acetyl-coenzyme A carboxylase and fatty acid synthetase after oestrogen treatment of the male chicken (Gallus domesticus). Int. J. Biochem., 21: 517-523.
CrossRefDirect Link - Martin-Castillo, A., M.T. Castells, G. Adanez, M.T.S. Polo, B.G. Perez and I. Ayala, 2010. Effect of atorvastatin and diet on non-alcoholic fatty liver disease activity score in hyperlipidemic chickens. Biomed. Pharmacother., 64: 275-281.
CrossRefPubMedDirect Link - Mezei, O., W.J. Banz, R.W. Steger, M.R. Peluso, T.A. Winters and N. Shay, 2003. Soy isoflavones exert antidiabetic and hypolipidemic effects through the PPAR pathways in obese Zucker rats and murine RAW 264.7 cells. J. Nutr., 133: 1238-1243.
PubMedDirect Link - Naaz, A., S. Yellayi, M.A. Zakroczymski, D. Bunick and D.R. Doerge et al., 2003. The soy isoflavone genistein decreases adipose deposition in mice. Endocrinology, 144: 3315-3320.
CrossRefPubMedDirect Link - Pearce, J. and D. Balnave, 1975. The effects of varying doses of oestradiol on liver lipid metabolism in the immature pullet. Biochem. Pharmacol., 24: 1843-1846.
CrossRefPubMedDirect Link - Pearson, A.W. and E.J. Butler, 1978. The oestrogenised chick as an experimental model for fatty liver-haemorrhagic syndrome in the fowl. Res. Vet. Sci., 24: 82-86.
PubMedDirect Link - Penza, M., C. Montani, A. Romani, P. Vignolini and B. Pampaloni et al., 2006. Genistein affects adipose tissue deposition in a dose-dependent and gender-specific manner. Endocrinology, 147: 5740-5751.
CrossRefPubMedDirect Link - Ratna, W.N., 2002. Inhibition of estrogenic stimulation of gene expression by genistein. Life Sci., 71: 865-877.
CrossRefPubMedDirect Link - Rimbach, G., C. Boesch-Saadatmandi, J. Frank, D. Fuchs and U. Wenzel et al., 2008. Dietary isoflavones in the prevention of cardiovascular disease: A molecular perspective. Food Chem. Toxicol., 46: 1308-1319.
CrossRefPubMedDirect Link - Saitoh, S., T. Sato, H. Harada and T. Takita, 2001. Transfer of soy isoflavone into the egg yolk of chickens. Biosci. Biotechnol. Biochem., 65: 2220-2225.
CrossRefPubMedDirect Link - Salih, S.M., P. Nallasamy, P. Muniyandi, V. Periyasami and A.C. Venkatraman, 2009. Genistein improves liver function and attenuates non-alcoholic fatty liver disease in a rat model of insulin resistance. J. Diabetes., 1: 278-287.
CrossRefPubMedDirect Link - Stake, P.E., T.N. Fredrickson and C.A. Bourdeau, 1981. Induction of fatty liver-hemorrhagic syndrome in laying hens by exogenous β-estradiol. Avian Dis., 25: 410-422.
PubMedDirect Link - Wagner, J.D., D.C. Schwenke, K.A. Greaves, L. Zhang, M.S. Anthony and R.M. Blair, 2003. Soy protein with isoflavones, but not an isoflavone-rich supplement, improves arterial low-density lipoprotein metabolism and atherogenesis. Arterioscler. Throm. Vas. Biol., 23: 2241-2246.
CrossRefPubMedDirect Link - Yang, H.Y., Y.H. Tzeng, C.Y. Chai, A.T. Hsieh, J.R. Chen, L.S. Chang and S.S. Yang, 2011. Soy protein retards the progression of non-alcoholic steatohepatitis via improvement of insulin resistance and steatosis. Nutrition, 27: 943-948.
CrossRefPubMedDirect Link