Irawan W. Kusuma
The United Graduate School of Agricultural Sciences, Ehime University,
3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan
Tomoko Ogawa
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi,
Matsuyama, Ehime, 790-8566, Japan
Kazutaka Itoh
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi,
Matsuyama, Ehime, 790-8566, Japan
Sanro Tachibana
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi,
Matsuyama, Ehime, 790-8566, Japan
ABSTRACT
An antifungal compound was isolated from the n-hexane solubles in methanolic extracts of amboyna wood by bioassay-guided fractionation using Pleurotus pulmonarius, a wood-rotting fungus. The compound was identified as β-eudesmol, a sesquiterpene alcohol with an eudesmane skeleton, based on instrumental analyses (Ultraviolet absorption, Mass spectrometry and 1H and 13C-Nuclear Magnetic Resonance spectroscopy) and a melting point test. The compound showed 21% of the antifungal activity of the methanolic extract as a control. Antifungal assays against P. pulmonarius showed that addition of β-eudesmol at different concentrations affected the inhibition of fungal growth. Furthermore, the activity to inhibit the growth of P. pulmonarius seemed to be dependent on the concentration of β-eudesmol.
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How to cite this article
Irawan W. Kusuma, Tomoko Ogawa, Kazutaka Itoh and Sanro Tachibana, 2004. Isolation and Identification of an Antifungal Sesquiterpene Alcohol from Amboyna Wood. Pakistan Journal of Biological Sciences, 7: 1735-1740.
DOI: 10.3923/pjbs.2004.1735.1740
URL: https://scialert.net/abstract/?doi=pjbs.2004.1735.1740
DOI: 10.3923/pjbs.2004.1735.1740
URL: https://scialert.net/abstract/?doi=pjbs.2004.1735.1740
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