Parisa Eshraghi
Department of Biology, Khatam University,
Reza Zarghami
Agricultural Biotechnology Researching Institute of Iran, Karaj, Mahdasht Road, P,O, Box 31535-1897, Iran
LiveDNA: 98.20698
Hamideh Ofoghi
Department of Biotechnology,
Iranian Research Organization for Science and Technology, P.O. Box 15815-3538, Tehran,
ABSTRACT
Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (R1-6) and mother plant in Iranian date palm (phoenix dactylifera L.) cultivar Khanizi. Total genomic DNA extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide Fermantas primers. Four primers of the set A to J primers were selected which revealed polymorphism and gave reproducible results. The genetic similarity between the mother plant and the callus-derived plantlets was ranged between 94% (for R1, R2) and 83% (for R5). Cluster analysis by the unweighted paired group method of arithmetic mean (UPGMA) showed a single large cluster at an estimated similarity coefficient (90.2%).
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How to cite this article
Parisa Eshraghi, Reza Zarghami and Hamideh Ofoghi, 2006. RAPD analysis of Micropropagated Plantlets in Date Palm. Pakistan Journal of Biological Sciences, 9: 111-114.
DOI: 10.3923/pjbs.2006.111.114
URL: https://scialert.net/abstract/?doi=pjbs.2006.111.114
DOI: 10.3923/pjbs.2006.111.114
URL: https://scialert.net/abstract/?doi=pjbs.2006.111.114
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