Jameel M. Al-Khayri
Date Palm Research Center,
Abdulaziz M. Al-Bahrany
Department of Horticulture, College of Agriculture and Food Sciences,
King Faisal University, P.O. Box 400, AI-Hassa 31982, Saudi Arabia
ABSTRACT
This study was conducted to establish an in vitro regeneration system for Hassawi rice (Oryza sativa L.), a land race cultivar adapted to eastern Saudi Arabia. The system consisted of two consecutive phases, callus induction and plant regeneration. Callus was established from mature caryopses cultured on MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.75, 1, 1.25, 1.5, 2, or 2.5 mg L-1 and 6-furfurylaminopurine (kinetin) at 0, 0.5, 1, or 2 mg L-1. Out of the explants, 30 to 80% formed callus depending upon hormonal combination, the remainder either germinated forming whole plants or produced roots only. Highest callus induction percentage occurred on 2.5 mg L-1 2,4-D alone; however, greatest callus weight was obtained on 1.5 mg L-1 2,4-D combined with 2 mg L-1 kinetin. To encourage regeneration, callus was transferred to hormone-free MS medium. Plant regeneration was best achieved, over 90%, from callus induced on 1 mg L-12,4-D with 0 or 0.5 mg L-1 kinetin, albeit these treatments were associated with relatively low callus frequency and small callus weight. Plantlets survived in soil and exhibited normal phenotype. This study has resulted in the first successful regeneration system for Hassawi rice and consequently has met the prerequisite for further biotechnological investigations relevant to this important crop.
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How to cite this article
Jameel M. Al-Khayri and Abdulaziz M. Al-Bahrany, 2000. In vitro Plant Regeneration of Hassawi Rice (Oryza sativa L.) From Mature Embryo-derived Callus. Pakistan Journal of Biological Sciences, 3: 602-605.
DOI: 10.3923/pjbs.2000.602.605
URL: https://scialert.net/abstract/?doi=pjbs.2000.602.605
DOI: 10.3923/pjbs.2000.602.605
URL: https://scialert.net/abstract/?doi=pjbs.2000.602.605
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