Asian Journal of Poultry Science1819-3609xxxx-xxxxAcademic Journals Inc.10.3923/ajpsaj.2021.24.30in vitro Medium]]>LestariKeri BabikianHaig BabikyanYusef MegantaraImam Kumar JhaRajeev KusumaArif S.W. Prayudhi NovantianaMargaretha Xaverius SudirmanFransiskus 12021151Background and Objective: Avian Infectious Bronchitis Virus (AIBV) is a highly infectious pathogen of chicken. There is no medication and vaccine developed yet due to its highly mutable nature. The objective was to formulate a blend that can be effective against the viral infection in chicken. Materials and Methods: Several natural essential oils, like, Gardenia jasminoides, Commiphora myrrha, Boswellia serrata, Foeniculum vulgare and Daucus carota with anti-viral properties were blended and tested against Avian Infectious Bronchitis Virus H120 (AIBV H120) strain using in vitro medium. The trial was conducted in an allantoic fluid medium. Different concentration of NOB was inoculated into the allantoic-fluid to challenge with the lethal dose of virus. Results: The Natural Oil Blend (NOB), at its minimum concentration up to 0.01 mL of 0.1%, was effective against the AIBV H120 strain. The NBO concentration lower than 0.1% was not sufficient to deactivate the AIBV H120 strain. The embryos up to 0.1% of NBO treated groups and negative control embryos were alive and tested negative to AIBV H120 strain, whereas the embryos in positive control died and tested positive. Conclusion: The in vitro trial proven that the essential oil Blend can deactivate the Avian Infectious Bronchitis Virus H120 (AIBV H120) in in vitro medium.]]>Enjuanes, L., I. Sola, S. Zuniga and F. Almazan,20082008pp: 149-202pp: 149-202Casais, R., B. Dove, D. Cavanagh and P. Britton,20037790849089Cavanagh, D.,200738281297Drosten, C., S. Günther, W. Preiser, S. van der Werf and H.R. Brodt et al.,200334819671976Ksiazek, T.G., D. Erdman, C.S. Goldsmith, S.R. Zaki and T. Peret et al.,200334819531966World Organisation For Animal Health (OIE),20182018OIE, A.,20086th Edn., pp: 1092-1106pp: 1092-1106Yang, Q., B. Wu, Y. Shi, X. Du and M. Fan et al.,2012Jasminoides ellis.]]>35917Chen, J.L., P. Blanc, C.A. Stoddart, M. Bogan and E.J. Rozhon et al.,1998Barleria prionitis with potent activity against respiratory syncytial virus.]]>6112951297Yunshi, Z., Y. Jing, Q. Xian, L. Xing, L. Xieqin and F. Ganzhu,2017Geniposide demonstrates anti-inflammatory and anti-viral activity against pandemic A/Jiangsu/1/2009 (H1N1) influenza virus infection in vitro and in vivo.]]>22599611Huang, A.G., X. Tu, X.Z. Qi, F. Ling, B. Zhu and G.X. Wang,2019Gardenia jasminoides Ellis inhibit white spot syndrome virus replication in red swamp crayfish Procambarus clarkii.]]>504239247Ndhlala, A.R., M. Moyo and J.V. Staden,20101569056930Gadir, S.A. and I.M. Ahmed,2014Commiphora myrrha and commiphora Africana essential oils.]]>6151156Mohamed, A.A., S.I. Ali, F.K. EL-Baz, A.K. Hegazy and M.A. Kord,2014in vitro antioxidant and antimicrobial activities of crude extracts of Commiphora myrrha resin.]]>571016Rhein, C., T. Weidner, L. Henβ, J. Martin, C. Weber and K. Sliva et al.,2015Boswellia serrata gum resin extract inhibit chikungunya and vesicular stomatitis virus infections in vitro.]]>1255157Shukla, H.S., P. Dubey and R.V. Chaturvedi,1989Foeniculum vulgare and Pimpinella anisum L.]]>9277279Brochot, A., A. Guilbot, L. Haddioui and C. Roques,20172017Jha, R.K., Y.H. Babikian, H.Y. Babikian, K.V. Le, D. Wisoyo, S. Srisombat and B. Jiaravanon,2017Vibrio parahaemolyticus in Penaeus vannamei.]]>216Jha, R.K., Y.H. Babikian, H.Y. Babikian, S.D. Wisoyo, Y. Asih, S. Srisombat and B. Jiaravanon,2016Penaeus vannamei.]]>2016Babikian, Y.H., H.Y. Babikian, R.K. Jha, S.D. Wisoyo, Y. Asih, S. Srisombat and B. Jiaravanon,2017Penaeus vannamei.]]>15056Babikian, H.Y., R.K. Jha, D.T.H. Oanh and T.Q. Phu,2019Penaeus vannamei) in an ahpnd bacterial challenge model.]]>5212217