International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2017.169.179MahmoudU.T. AmenO.A. ApplegateT.J. ChengH.W. 52017165Objective: This study aimed to examine the effect of dietary Brazilian propolis on the growth performance, physiological homeostasis and gut characteristics in broiler chickens reared under mild chronic heat stress. Materials and Methods: Five hundred and four 15 days old male broiler chicks were fed one of six diet (0.0, 100, 250, 500, 1000 and 3000 mg kg1 propolis). Growth performance was evaluated in terms of Body Weight (BW), Body Weight Gain (BWG), Feed Intake (FI) and Feed Conversion Ratio (FCR) at 2 weeks intervals to 42 day of age. At 42 day of age 12 birds from each group were randomly selected and sacrificed for determination of the relative weight of internal organs and cecal contents were collected for microbial enumeration. Duodenal, jejunal and ileal tissue samples were collected for measuring villus height and width, crypt depth and villus crypt ratio. Also, blood was collected for subpopulations of leukocytes counts and serum chemical and hormonal analysis. In addition, brain samples were collected for determination of the heat stress-induced changes of the Heat Shock Protein 70 (HSP70) gene expression. The data were analyzed by one-way analysis of variance using the General Linear Models (GLM) procedure. Results: The results indicated that dietary propolis supplementation had no effect on growth performance and liver, heart, gizzard and spleen weights (p>0.05). While, compared to controls, the abdominal fat weight was increased with propolis supplementation (p = 0.035). Propolis did not affect cecal concentrations of Escherichia coli, total coliforms, Enterococcus spp. and total lactobacilli (p>0.05). However, compared to controls, the Bifidobacterium spp., population was lower in birds fed diet with propolis at 1000 mg kg1 (p = 0.005). Propolis had no effect (p>0.05) on intestinal villus height and width, crypt depth and villus:crypt ratio. Compared to controls, propolis dietary supplementation did not affect the populations of eosinophils, monocytes and basophils; and serum concentrations of total proteins, globulins, phosphate, calcium, glucose and thyroid hormones as well as HSP70 mRNA expression in brain tissues (p>0.05, respectively). However, propolis regardless of dose reduced the number of heterophils, heterophil:lymphocyte ratio (H/L) and serum corticosterone and aminotransferase (AST) concentrations (p<0.05, respectively). In addition, all doses of propolis, except for 100 mg kg1, significantly increased circulating lymphocytes and reduced uric acid concentrations. In addition, there was an effect of propolis on serum albumin and tri-iodothyronine:thyroxin (T3/T4) ratio. Compared to the control group, birds fed 250 mg kg1 propolis had a significantly higher T3/T4 ratio; while both 100 and 3000 mg kg1 propolis groups had significantly increased the serum albumin concentrations. Conclusion: It is concluded that dietary supplementation of green Brazilian propolis at the tested doses, improves health status of birds by reducing initiation of heat stress responses, such as reduced concentrations of corticosterone, H/L ratio, AST and uric acid and increased T3/T4 ratio.]]>Tawfeek, S.S., K.M.A. Hassanin and I.M.I. Youssef,201441019Pawar, S.S., S. Basavaraj, L.V. Dhansing, K.N. Pandurang and K.A. Sahebrao et al.,20164332341Baziz, H.A., P.A. Geraert, J.C.F. Padilha and S. Guillaumin,199675505513Cooper, M.A. and K.W. Washburn,199877237242Yalcin, S., P. Settar, S. Ozkan and A. Cahaner,199776921929Ramnath, V., P.S. Rekha and K.S. Sujatha,200857784Yalcin, S., S. Ozkan, M. Cabuk and P.B. Siegel,200312382388Abaseikong, S.F.,198721114Yahav, S., A. Straschnow, I. Plavnik and S. Hurwitz,199776627633Abd El-Gawad, A.H., A.E.A. Hemid, I. El-Wardany, E.F. El-Daly and N.A.A. El-Azeem,20084605611Niu, Z.Y., F.Z. Liu, Q.L. Yan and W.C. Li,20098821012107St-Pierre, N.R., B. Cobanov and G. Schnitkey,200386E52E77Park, S.O., J. Hwangbo, C.M. Ryu, B.S. Park and H.S. Chae et al.,2013IgG and cecum microflora of broiler chickens.]]>1512041208Imik, H., O. Kaynar, S. Ozkanlar, R. Gumus, H. Polat and Y. Ozkanlar,20131645259Zulkifli, I., A. Al-Aqil, A.R. Omar, A.Q. Sazili and M.A. Rajion,200988471476Lin, H., E. Decuypere and J. Buyse,20061441117Sahin, N., K. Sahin and O. Kukuk,200146286292Sahin, K., N. Sahin, M. Sari and M.F. Gursu,200222723731Sahin, K., O. Kucuk, N. Sahin and M. Sari,20027291100Akbarian, A., A. Golian, H. Kermanshahi, R. Farhoosh, A.R. Raji, S. de Smet and J. Michiels,201311109119Falowo, A.B., P.O. Fayemi and V. Muchenje,201464171181Bankova, V., M. Popova and B. Trusheva,20142014Abdel-Mohsein, H.S., M.A. Mahmoud and U.T. Mahmoud,20142204211Seven, I., T. Aksu and P.T. Seven,20102314821489Seven, P.T. and I. Seven,200834193196Seven, T.P., I. Seven, M. Yilmaz and U.G. Simsek,2008146137148Mahmoud, U.T., M.A. Abdel-Rahman and M.A. Hosny,201341827Mahmoud, U.T., M.R. Fahmey, M.A. Abdel-Rahman and M.H.A. Darwish,20144117122Cheng, H.W., G. Dillworth, P. Singleton, Y. Chen and W.M. Muirt,20018012781285Corrons, J.L.V., S. Albarede, G. Flandrin, S. Heller and K. Horvath et al.,200442922926Campbell, T.W.,19952nd Edn.,Pages: 108Pages: 108Kuenzel, W.J. and M. Masson,1988Gallus domesticus).]]>Pages: 166Pages: 166Felver-Gant, J.N., L.A. Mack, R.L. Dennis, S.D. Eicher and H.W. Cheng,20129115421551Yu, J. and E. Bao,20082111161126Sieuwerts, S., F.A.M. de Bok, E. Mols, W.M. de Vos and J.E.T. van Hylckama Vlieg,200847275278Hori, J.I., D.S. Zamboni, D.B. Carrao, G.H. Goldman and A.A. Berretta,20132013Mahmoud, U.T., M.A. Abdel-Rahman and M.H. Darwish,20133154160Hassan, M.G. and T.A. Abdulla,2011257782Kouba, M., D. Hermier and J. Le Dividich,2001798187Lu, Q., J. Wen and H. Zhang,20078610591064Pascual, C., R. Gonzalez and R.G. Torricella,199441913Gross, W.B. and H.S. Siegel,198327972979Altan, O., A. Altan, M. Cabuk and H. Bayraktar,200024145148Galal, A., A.M. Abd-El-Motaal, A.M.H. Ahmed and T.G. Zaki,20087272278Khan, W.A., A. Khan, A.D. Anjum and Zia-ur-Rehman,200247475Hosseini-Vashan, S.J., A. Golian, A. Yaghobfar, A. Zarban, N. Afzali and P. Esmaeilinasab,2012111611816125Attia, Y.A., A.E. Abd Al-Hamid, M.S. Ibrahim, M.A. Al-Harthi, F. Bovera and A. El-Naggar,20141648795Lierz, M.,200362955Harsini, S.G., M. Habibiyan, M.M. Moeini and A.R. Abdolmohammadi,2012148322330Yoshizumi, K., N. Nishioka and T. Tsuji,2005125315321, (In Japanese)Azab, A.E.S., F.A. Fetouh and M.O. Albasha,201422835Denli, M., S. Cankaya, S. Silici, F. Okan and A.N. Uluocak,2005Coturnix coturnix Japonica).]]>18848854Babinska, I., K. Kleczek, W. Makowski and J. Szarek,20133314Lee, M.S., Y.H. Kim, W.S. Park, W.G. Ahn and O.K. Park et al.,20132013Gao, Y.J. and R.R. Ji,200921117Beyzai, A.R. and M. Adibmoradi,20111014961501Tekeli, A., H.R. Kutlu and L. Celik,2011Z. officinale and propolis extracts on the performance, carcass and some blood parameters of broiler chicks.]]>11223Kacaniova, M., P. Hascik, L. Hleba, J. Pochop and M. Melich et al.,20115372376Krocko, M., M. Canigova, J. Bezekova, M. Lavova, P. Hascik and V. Duckova,2012456367Haddadin, M.S.Y., I. Nazer, S.J.A. Raddad and R.K. Robinson,20087391394Yamauchi, K., T. Buwjoom, K. Koge and T. Ebashi,200685645651Eyng, C., A.E. Murakami, C.R.A. Duarte and T.C. Santos,201498393401Tekeli, A., H.R. Kutlu, L. Celik and F. Doran,2010Z. officinale and propolis extracts on intestinal microbiology and histological characteristics in broilers.]]>9898906Marcucci, M.C.,1995268399