International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2018.348.355GunawanAsep NurajizahEva Siti ListyariniKasita FurqonAhmad BilyaroWoki SumantriCece Jakaria Hasina AkterSyeda Jasim UddinMuhammad 72018177Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.]]>Yu, K., G. Shu, F. Yuan, X. Zhu and P. Gao et al.,20139108118Cameron, N.D., M. Enser, G.R. Nute, F.M. Whittington and J.C. Penman et al.,200055187195Furqon, A., A. Gunawan, N. Ulupi, T. Suryati and C. Sumantri,201740151157Prasad, M.R. and V.C. Joshi,197925463626368Maharani, D., H.B. Park, J.B. Lee, C.K. Yoo and H.T. Lim et al.,2013407380Matsuhashi, T., S. Maruyama, Y. Uemoto, N. Kobayashi and H. Mannen et al.,2011891222Ohsaki, H., A. Tanaka, S. Hoashi, S. Sasazaki and K. Oyama et al.,200980225232Taniguchi, M., T. Utsugi, K. Oyama, H. Mannen and M. Kobayashi et al.,200414142148Uemoto, Y., T. Abe, N. Tameoka, H. Hasebe and K. Inoue et al.,201042141148Folch, J., M. Lees and G.H.S. Stanley,1957226497509Jeong, J., X. Shi, X. Zhang, S. Kim and C. Shen,20112011Gunawan, A., K. Kaewmala, M.J. Uddin, M.U. Cinar and D. Tesfaye et al.,20111281121Rozen, S. and H. Skaletsky,2000132365386Silver, N., S. Best, J. Jiang and S.L. Thein,20062006Cinar, M.U., A. Kayan, M.J. Uddin, E. Jonas and D. Tesfaye et al.,20123948094821Kayan, A., M.U. Cinar, M.J. Uddin, C. Phatsara and K. Wimmers et al.,2011897683Nestel, P., P. Clifton and M. Noakes,199435656662Grundy, S.M.,199460986S990SWarensjo, E., J. Sundstrom, B. Vessby, T. Cederholm and U. Riserus,200888203209Lu, P., D. Li, J. Yin, L. Zhang and Z. Wang,200810715291537Tapiero, H., G.N. Ba, P. Couvreur and K.D. Tew,200256215222Gebauer, S.K., T.L. Psota, W.S. Harris and P.M. Kris-Etherton,200683S1526S1535Mauvoisin, D. and C. Mounier,2011937886Merino, D.M., D.W.L. Ma and D.M. Mutch,20102010Patton, K.K., P.T. Ellinor, S.R. Heckbert, R.H. Christenson, C. DeFilippi, J.S. Gottdiener and R.A. Kronmal,200912017681774Nitambi, J.M. and M. Miyazaki,20044391104Miyazaki, M. and J.M. Ntambi,200368113121Hulbert, A.J.,201050808817Uemoto, Y., H. Nakano, T. Kikuchi, S. Sato and M. Ishida et al.,2011SCD gene effects on fatty acid composition and melting point of fat in a Duroc purebred population.]]>43225228Ren, J., C. Knorr, L. Huang and B. Brenig,20043401930Quintanilla, R., R.N. Pena, D. Gallardo, A. Canovas and O. Ramirez et al.,20118929632971Sanchez, M.P., N. Iannuccelli, B. Basso, J.P. Bidanel and Y. Billon et al.,20072007Dobrzyn, P., A. Dobrzyn, M. Miyazaki, P. Cohen and E. Asilmaz et al.,200410164096414Mashek, D.G. and R.A. Coleman,200617274278