Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.1998.170.174Gossypium hirsutum L.) With Insect Resistant Gene by Particle Bombardment and Agrobacterium]]>A.A. HarisWasif. HusnainT. RiazuddinS. 3199813A combination of two techniques i.e. particle bombardment and Agrobacterium method was applied to transfer marker NPT-II and insecticidal cry1A(b) gene into zygotic embryos of cotton variety MNH-93. Wounding in embryos produced by bombardment of tungsten particles increased the efficiency of Agrobacterium mediated transformation. Primary transformants selected on selection medium containing Kanamycin (100 μg/ml) for 6-8 week showed the presence and expression of cry1A(b) gene in the primary transformants. Insect Bioassays with 2nd instar larvae of of American bollworm (Heliothis armigera), PCR amplification of the coding region of cry1A(b) gene in plant genome and ELISA with the antibodies against cry1A(c) confirmed the transformation.]]>Altman, D.W., J.H. Benedict and E.S. Sachs,1996Bacillus thuringiensis Genes.]]>792106114Van Aarssen, R., P. Soetaert, M. Stam, J. Dockx and V. Gossele et al.,1995cry IA(b) transcript formation in tobacco is inefficient.]]>28513524Doyle, J.J. and J.L. Doyle,1987191115Draper, J., R. Scott, P. Armitage and R. Walden,1988Engvall, E. and P. Perlmann,19718871874Finer, J.J. and M.D. McMullen,1990Gossypium hirsutum L.) via particle bombardment.]]>8586589Firoozabady, E., D.L. DeBoer, D.L. Merlo, E.L. Halk, L.N. Amerson, K.E. Rashka and E.E. Murray,1987Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants.]]>10105116Frederick, M., R. Ausubel, R. Brent, E. Kingstem and D.D. Moore et al.,1989Perlak, F.J., R.W. Deaton, T.A. Armstrong, R.L. Fuchs, S.R. Sims, J.T. Greenplate and D.A. Fischhoff,19908939943Fujimoto, H., K. Itoh, M. Yamamoto, J. Kyozuka and K. Shimamoto,1993Bacillus thuringiensis.]]>1111511155Hashmi, L.A., M.H. Qazi and P. Khaliq,199515715Benedict, J.H., E.S. Sachs, D.W. Altman, W.R. Deaton, R.J. Kohel, D.R. Ring and S.A. Berberich,199689230238Bidney, D., C. Scelonge, J. Martich, M. Burrus, L. Sims and G. Huffman,1992Agrobacterium tumefaciens.]]>18301313Chlan, C.A., J. Lin, J.W. Cray and T.E. Cleveland,1995133137Christou, P.,199329119124Cousins, Y.L., B.R. Lyon and D.J. Llewellyn,199118481494Husnain, T., F. Khanum, S. Riazuddin and M.P. Gordon,1995Oryza sativa L.) with bacterial genes by particle bombardment.]]>1219228John, M.E. and G. Keller,1996931276812773Knittel, N., V. Gruber, G. Hahne and P. Lenee,1994Helianthus annuus L.): A reliable protocol.]]>148186McCabe, D.E. and B.J. Martinell,19911991Murashige, T. and F. Skoog,196215473497Sims, S.R. and S.A. Berberich,199689247251Umbeck, P., G. Jahnsan, K. Barten and W. Swain,1987Gossypium hirsutum L.) plants.]]>5263266