Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.1098.1099PervezKhalid 6200036All 50 blood samples from Chronic Liver Disease (CLD) patients were screened for (Antibodies against hepatitis C virus) Anti HCV by 3rd generation (Enzyme Linked Immuno-Sorbant Assay) ELISA for diagnosis and genotyping of HCV by (Polymerase Chain Reaction) PCR. Samples were screened by slot blot hybridization using P32 ATP labelled genotype specific oligonucleotide probes indicating prevalence of genotype HCV1b which was further confirmed by southern hybridization and direct sequencing. Positivity by ELISA was 37% while with that of PCR was 42% in hepatitis patients indicating its sensitivity and reliability and can be used to determine the carrier state and hence its spread can be checked through blood screening.]]>Alberti, A., P. Pontisso, L. Chemello, G. Fattovich, L. Benvegnu, F. Belussi and M.S. de Mitri,1994223841Camps, J., J. Cordoba and J.I. Esteban,199543691702Gane, E.J., B.C. Portmann, N.V. Naoumov, H.M. Smith and J.A. Underhill et al.,1996334815821Davis, L.G., W.M. Kuehl and J.F. Battey,19942nd Edn.,pp: 159-161pp: 159-161Dusheiko, G.M.,1994344605606Hess, G.,199440905911Justin, M.,19951322930Petrelli, E., A. Manzin, S. Paolucci, A. Cioppi, M. Brugia, P. Muretto and M. Clementi,199447148151Stanford, T. and S.T. Shulman,19924th Edn.,pp: 312-326pp: 312-326Stuyver, L., R. Rossau, A. Wyseur, M. Duhamel, B. Vanderborght, H. van Heuverswyn and G. Maertens,19937410931102Tisminetzky, S.G., M. Gerotto, P. Pontisso, L. Chemello, M.G. Ruvoletto, F. Baralle and A. Alberti,19942105112Van der Poel, C.L., H.W. Reesink and H.T. Cuypers,199434414751479Zekri, A.R.N., A. Bahnassy, H.M. Khaled, O. Mansour and A.M. Attiam,19958331335