Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.416.418In vitro and Field Grown Plants of Rauvolfia serpentina]]>AhmadShahrear AminM. N.MosaddikM. Ashik4200254Phytochemical screening and alkaloid estimation of in vitro and field grown plants of R. serpentina has been investigated. Under the phytochemical screening moisture content (at 45 and 105oC), biomass yield and alkaloid content in vitro and field grown plant materials were determined. At 45oC in vitro plant materials (leaf, root and callus) have higher moisture content compared with field grown (3 years mature plants) plant materials (leaf and root). But opposite result was observed at 105oC. Percent yield of crude plant extract (Biomass yield) of in vitro plant materials was higher than that of field grown plant materials. The percentage of alkaloid for in vitro plant materials was 1.17% in callus, 0.98% in root and 0.49% in leaf, respectively. On the other hand it was found that the field grown plant materials were 0.71% in leaf and 1.43% in root, respectively.]]>AOAC.,1984Ahmad, S., M.N. Amin, M.A.K. Azad and M.A. Mosaddik,2002Rauvolfia serpentina by tissue culture technique.]]>57579Ghani, A.,19982nd Edn.,pp: 276pp: 276Harkes, P.A.A., L. Krijbolder, K.R. Libbenga, R. Wignsma and R. Verpuorte,1985Cinchora ledgerriana tissue cultures and the production of alkaloids and anthraquinones therin.]]>4199214Illahi, I.,1993Rauvolfia cell culture.]]>231921Nikolaeva, L., H. Sergeeva, V. Kuznetsova and E. Korbelainen,1996Rauvolfia serpentina tissue culture as a raw material for obtaining antiarrhythmic preparations.]]>1217217Payne, J., J.D. Hamill, R.J. Robines and M.J.C. Rhodes,1987Datura stramonium.]]>53474478Verpoorte, R., R. Wijnsama, T.H. Mulder, B.K. Krieger, P.A.A. Harkes and A.S. Baerheim,1985Cincona Species.]]>1985pp: 196-208pp: 196-208WHO.,1996pp: 224pp: 224Wijnsma, R., R. Verpoorte, P.A.A. Harkes, H.J.G.T. Hoopen, J.J. Meiger and W.M. Van Gulie,19871987pp: 485-494pp: 485-494Wink, M.,19871987pp: 17-42pp: 17-42Sergeeva, H., V. Kuznetsova, E. Korbelainen, L. Nikolaeva and S. Minina,19963303304