Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.693.695Brassica napus Cv. Oscar]]>KhanMuhammad RamzanRashidHamid QuraishiAzra 6200256Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.]]>De Block, M., D. Debrouwer and P. Tenning,1989Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants.]]>91694701Dietert, M.F., S.A. Barron and O.C. Yoder,1982in vitro culture in the genus Brassica.]]>26233240Dodds, J.H. and L.W. Roberts,1982pp: 21-35pp: 21-35Dunwell, J.M.,1981In vitro regeneration from excised leaf discs of Brassica species.]]>32789799Gamborg, O.L., R.A. Miller and K. Ojima,196850151158Jha, T.B. and S.C. Roy,1982Vicia fave.]]>47465470Klimaszewska, K. and W.A. Keller,1985Brassica napus.]]>4183197Knutzon, D.S., G.A. Thompson, S.E. Radke, W.B. Johnson, V.C. Knauf and J.C. Kridl,1992Brassica seed oil by antisense expression of a stearoly acyl carrier protein desaturase gene.]]>8926242628Moloney, M.M., J.M. Walker and K.K. Sharma,1989Brassica napus using Agrobacterium vectors.]]>8238242Murashige, T. and F. Skoog,196215473497Murata, M. and T.J. Orton,1987Brassica species.]]>11111123Narasimhulu, S.B. and V.L. Chopra,1988Brassica.]]>7104106Phogat, S.K., P.K. Burma and D. Pental,2000Brassica napus varieties and genetic transformation of stocks containing fertility restorer genes for two cytoplasmic male sterility systems.]]>97379Pua, E.C., A. Mehra-Palta, F. Nagy and N.H. Chua,1991Brassica napus L.]]>5815817Riemenschiender, D.F., B.E. Haissig and E.T. Bingham,1988pp: 433-494pp: 433-494Sharma, K.K. and T.A. Thorpe,1989In vitro regeneration of shoot buds and plantlets from seedling root segments of Brassica napus L.]]>18129141Shi, S.W. and Y.M. Zhou,1998Brassica napus).]]>2016Stringam, G.R.,1977Brassica napus L.]]>9115119