Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.80.83Corneybacterium glutamicum ]]>ShahAbdul Haleem HameedAbdul KhanGul Majid 1200251The objective of this investigation was to develop a new auxotrophic mutant from a regulatory mutant and to examine its potency for lysine production. Glutamate-homoserine-alanine triple auxotrophic mutant MRLH-GHA10 was derived from thiosine resistant mutant of Corneybacterium glutamicum. Its potency was examined in various media containing different carbon sources. The most potent auxotrophic mutant developed in this investigation produced 38, 33 and 28.5 g L-1 lysine, in the media containing glucose, molasses and starch hydrolyzate, respectively as carbon sources in a stirred tank fermenter, at 30 °C and at 500 rpm agitation.]]>Aiko, R., R. Ishiwata and S. Maeda,197124335337Davis, B.D. and E.S. Mangioli,1950162534536How. E., G.R. Jansen and E.W. Gillfillam,196516315320Hsiao, T.Y. and C.E. Glatz,199649341345Ishino, S., K. Yamaguchi, K. Shirahata and K. Araki,19844825572560Joseph, A., P.R. Tasker, K. Joseph, N.N. Rao and M. Swaminathan et al.,196222113117Kinnoshirta, S., K. Tanaka and S. Akida,1960Corneybacterium glutamicum.]]>34589593Momose, H. and T. Takagi,19474219111917Pelechova. J, F. Smekal, V. Koura, J. Plachy and V. Krumphenzi,198325341346Plachy, J., J. Pelzbener, V. Bulant and F. Palechova,1988Brevibacterium flevum.]]>347071Saito, H and Y. Ikada,19573240249Yamaguchi, K., S. Ishino, K. Aruki and K. Shirahata,19865024532459Bridge, B.A.,19761976pp: 16Nakayama, K., K. Sagamihara and K. Araki,19851985Shah, A.H. and A. Hameed,19991999Sikyta, B.,1983pp: 97pp: 97