Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2003.1435.1441Artocarpus integrifolis) as a Source of Dairy Enzyme
I. Isolation, Partial Purification and Some Properties]]>Al-TanbolyAl-Sayed 122003616The aim of the present work was to search for a novel plant proteinase enzyme from Jack fruit
(Artocarpus integrifolis) as a source of dairy enzymes that would be natural products which can be
easily extracted at relatively low cost and no legal barriers. This enzyme was subjected to a
purification scheme composed of ammonium sulfate fractionation followed by gel filtration on G-100 Sephadex column. The enzyme was purified 2.70-fold with a total yield of 23.77% of the
original activity. There were relationship between temperature and incubation time, the enzyme
activity increase was observed up to 55°C for 60 min reaction time and still constant thereafter.
Proteinase was active over a broad temperature range retained about 37.4 and 24.9% of temperature
activity at 35 and 80°C for 5 and 60 min. An energy of activation of 9.98 KJ mole‾1 for the enzyme
activity was derived from the Arrhenius plot of initial velocity (Vo) across a temperature ranging
from 40 to 55°C. The optimum pH was pH 7.5. The rate of thermal inactivation proceeded more
rapidly at pH 7.0 and 8.0, when heating at 50°C for 60 min the enzyme activity lost about 95 and
92% its activity, respectively. Michaelis-constant of (Km) values of 2.0 mg ml‾1 and a maximum
initial velocity (Vmax) of 0.75 µ moles mg‾1 when casein used as a substrate. A Molecular weight
(MW) determination of ~22 kDa was estimated by gel filtration methods using a Sephadex G-100.
Cu2+, K2+ , Fe2+ and Zn2+strongly inhibited the enzyme. However, Ca++ slightly stimulated. EDTA,
sodium azide, Sodium citrate and urea among the chemical reagents inhibited the proteinase activity.]]>Andrews, P.,196411222227Cousin, M.A.,198245172207El-Tanboly, E.,2001Durio zibethinus). I. Isolation and partial characterization.]]>1215311534Fairbairn, D.J. and B.A. Law,198653139177Fernandez, J., A.F. Mohedano, P. Gaya, M. Medina and M. Nunez,1999Pseudomonas flurorescens INIA 745, an Isolate from Ewe's milk.]]>5543546Koka, R. and B.C. Weimer,2000Pseudomonas flurorescens RO98.]]>89280288Goodenough, P.W. and J. Owen,1987267479Gomori, G.,19551138146Greenberg, D.M.,195725464Gupta, R., Q.K. Beg and P. Lorenz,2002591532Hyekeong, P., I. Kusakabe, Y. Sakakibara and H. Kobayashi,200165702705Ismail, A., A.E. El-Tanboly and N.S. Abd-ul-Rabou,1999Artocarpus integrifolis) as a source of dairy enzyme.]]>1999pp: 7373Lineweaver, H. and D. Burk,193456658666Ohnistti, S.T. and J.K. Barr,197886193200Maksimenko, A.V., L.A. Nadirashvili, A.D. Romashchenk, G.S. Erkomaishvili and V.P. Torchilin,199025760Matta , H., V. Punj, M.S. Kalra,1994Pseudomonas sp. AFT-36.]]>49186189Montes, C., M. Amador, D. Cuevas and F. Cordoba,1990Bromelia plumieri (karatas).]]>541724Mottar, J.F.,19891989pp: 227-239pp: 227-239Shenolikar, S. and K.J. Stevenson,1982Humicola lanuginosa.]]>205147152Muderriszade, A., N.Y. Ensari, S. Aguloglus and B. Otudil,2001Bacillus sp.]]>37574577Seong-II, L., K. Eun-Jung, C. Shin-Yang and Y.J. Yong,2002Rhizopus stolonifer, corymbifera Rhizopus oryzae and Absidia corymbifera from Korean traditional meju.]]>31211215Silva, S.S. and F.X. Malcata,199967373378Stepaniak, L., P.F. Fox and C. Daly,1982Pseudomonas fluorescens AFT36.]]>717376384Venables, C.E. and S.C. Watkinson,1989513691374White, C.H. and R.T. Marshall,1973Pseudomonas fluorescens P26.]]>56849853Zherebtsov, N.A. and L.V. Shcheblykina,1983Rhizopus pygmaeus.]]>29226231Zimacheve, A.V., E.V. Lyevleva, P.H. Huan, V.H. Nhan and V.V. Mosolov,1994Ananas comosus L.).]]>30215222Baral, A., P.F. Fox and T.P. O'Connor,1995Pseudomonas tolaasii.]]>39757762Kaplan, C.,1955Hartley, B.S., 1960294572Mckellar, R.C. and M. Cholette,19856832163223Mitchell, S.L. and R.T. Marchall,198972875882White, J.S. and D.C. White,1997Azarkan, M., M. Nijs, N. Smolders, J. Guermant, J. Vlncentelli and Y. Looze,199660167183Dolidze, J.A., V.S. Berikashvili, T.S. Dvali and G.I. Kvesitadze,19824349355