Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2003.1698.1705AnwarKhurshid 122003619Present study was designed to investigate the toxic effects of 50, 100, 200 and 400 ppm cypermethrin administered as a single dose at 0 day of incubation, on the development of chick at day 7 of incubation. The study included the investigation of teratological and biochemical changes in the developing embryos. Among biochemical constituents, activities of a few enzymes and some biochemical contents of the whole embryo were investigated. Activities of the enzymes amylase and AkP were affected with cypermethrin treatment. Activity of amylase increased whereas the activity of AkP decreased. Amylase activity was elevated at 400 ppm by 762%. In contrast, the activity of AkP was inhibited at 100, 200 and 400 ppm by 32, 85 and 53%, respectively. However, the activities of AcP, ALT, AST and LDH remained unaltered. Of the biochemical components, glycogen, free amino acids, total lipids, cholesterol, DNA and RNA contents were seriously affected. Total protein, soluble protein, uric acid and urea contents also remained unaffected with cypermethrin treatment. Cypermethrin treatment had no effect on embryonic glucose content, whereas, it affected glycogen content of the whole embryo in non-consistent manner. Glycogen content was increased at 50, 200 and 400 ppm and decreased at 100 ppm. Free amino acid content was decreased at 100, 200 and 400 ppm. Cholesterol content showed a significant increase at 200 ppm. Total lipid content was increased at 200 ppm and decreased at 400 ppm. DNA was increased at 200 ppm and decreased at 400 ppm, while RNA showed change only at the dose of 50 ppm. Teratological changes observed in the present study included the reduction in crown rump length, the size of brain and the size of eyeballs, incomplete development of eyes, beak and wing buds, micromelia, exocardiogenesis. In some treatment groups, eyes and in some groups beaks were totally absent.]]>Akhtar, M.H., C. Danis, H.L. Trenholm and K.E. Hartin,199227235253Amer, S.M. and E.I. Aboul-Ela,1985155135142Cabaud, P.G. and F. Wroblewski,195830234236Carraway, W.T.,1963pp: 239pp: 239Clemedson, C., B. Schmid and E. Walum, 1989in ovo. ]]>3271275Consolazio, C.F. and J.M. Lacono,19631963pp: 317-367pp: 317-367Das, B.K. and S.C. Mukherjee,2003Labeo rohita fingerlings: Biochemical, enzymatic and haematological consequences.]]>134109121Eells, J.T., J.L. Rasmussen, P.A. Bandettini and J.M. Propp,1993123107119Eells, J.T., P.A. Bandettini, P.A. Holman and J.M. Propp,199226211731181Elbetieha, A., S.I. Da'as, W. Khamas and H. Darmani,200141522528El-Demerdash, F.M., M.I. Yousef and K.S. Al-Salhen,200338365378El-Tawil, O.S. and M.S. Abdel-Rahman, 2001443340El-Toukhy, M.A. and R.S. Girgis,1993In vivo and in vitro studies on the effect of larvin and cypermethrin on adenosine triphosphatase activity of male rats.]]>28599619Giray, B., A. Gurbay and F. Hincal,2001118139146Ghosh, T.K.,1989Labeorhita.]]>55115119Giri, S., A. Giri, G.D. Sharma and S.B. Parsed, 2003in vivo. ]]>185358Gowland, B.T.G., C.F. Moffat, R.M. Stagg, D.F. Houlihan and I.M. Davies,2002Carcinus maenas.]]>54169177Hamburger, V. and H.L. Hamilton,1951884992Haratym-Maj, A., 20029199206Hartel, A., R. Helger and H. Lang,19697183184Heder, A.F., K.I. Hirsch-Ernst, D. Bauer, G.F. Kahland and H. Desel,2001627179Henry, R.J. and M. Henry,1974Institoris, L., U. Undeger, O. Seroki, M. Nehez and I. Desi,19991374755Izaguirre, M.F., R.C. Lajmanovich, P.M. Peltzer, A.P. Solar and V.H. Casco,2000Physalaemus biligonigerus tadpoles (Anura: Leptodactylidae).]]>65501507Kapoor, R.K., S.S. Chauhan, N. Sing and U.K. Misra,198832205211Kind, P.R.N. and E.J. King,19547322326Majmunder, S., A.K. Chakraborty, T.K. Mandal, A. Bhattacharya and K. Basak,199432752756Misawa, M., J. Doul, P.A. Kitose and E.M. Uyeky, 1981572029Moore, S. and W.H. Stein,1954211907913Mufti, S.A. and R. Nasim,198733109121Natelson, S., M.L. Scott and C. Beffa,195121275281Onikienko, E.A.,19631963Reddy, A.T. and K. Yellamma, 1991Tilapia mossambica.]]>23633638Reitman, S. and S. Frankel,1957285663Santoni, G., F. Cantalamessa, L. Mazzucca, S. Romagnoli and M. Piccoli, 1997120231242Sarafian, T.A. and D.E. Bredesen, 19942118Schneider, W.C.,19571957pp: 680-684pp: 680-684Shakoori, A.R. and M.S. Ahmed,1973Gallus domesticus. I. Liver growth and nucleic acids content.]]>5111117Sheela, M. and S. Muniandi, 1992Lepidocephalichthyes thermalis.]]>10829832Sheets, L.P.,2000215763Sibley, P.K. and N.K. Kaushik,199120168176Simon, L.M., K. Laszlo, M. Kotorman, A. Vertesi, K. Bagi and J. Nemcsook,1999Cyprinus carpio L.).]]>34819828Surralles, J., N. Xamena, A. Creus, J. Catalan, H. Norppa and R. Macros,1995341169184Tonini, M., L.G. Costa, S.M. Candura, G. Olibet, C.A. Rizzi, L. Garlaschelli and L. Manzo,198910707715Usmani, K.A and C.O. Knowles, 2001Helicoverpa zea, Spodoptera frugiperda and Agrotis ipsilon (Lepidoptera: Noctuidae). ]]>94868873Van Steenis, G. and M.J. van Loughten,197119675686Walker, N.E., 197119590601Wootton, I.D.P. and H. Freeman,1964Zollner, N. and K. Kirsch,1962135545561Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275