Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.1995.1998in vitro Propagation Techniques of Taxus (Taxus media)]]>HongboShao ShuiyuanJiang LiyeChu ChangxingZhao JinminWei BiaoLi QingjieGuo 102006910In this study, a primary investigation on in vitro propagation technique of Taxus (Taxus media) for the sake of improving the eco-environment and increasing economic effect in west China zone was conducted. The better result was obtained and a relatively simple and practical procedure of In vitro propagating of Taxus (Taxus media) was established, which has a broad prospect in improving eco-environment and promotion of economy in west China region. The related results were as followed: The 2 year branches of Taxus media could be selected as explants, obtaining 78% of budding and transplanting survival rate reaching 90%. Use of 0.1% HgCl2 for sterilization for 12 m could reach more than 90% effect of sterilization, which was better than that previous reported. The flowchart for effective in vitro propagation of Taxus media under our experimental condition was below: recover and initiation of explants →bud propagation →subculture →rooting →transplanting in greenhouse →transplanting in fields or going to market.]]>Chen, Y.Q., W.H. Zhu and W.Q. Wu et al.,2000Taxus and difference in Taxol content.]]>31216218Chen, Z.S. and Q. Wang,2001Taxus chinensis.]]>21259261Chaves, M.M., J.P. Maroco and J.S. Pereira,200330239264Chu, C.C.,20024410751084Dellapenna, D.,2001125160163Gan, F.Y., L.P. Peng and G.Z. Zheng,1996Taxol in cell culture of Taxus yunnansis. Acta Yuannan.]]>18451453Gan, F.Y., Y.M. Shen and X.J. Hao,1999Taxus biotechnology.]]>176574Gan, F.Y., Y.M. Shen and X.J. Hao,2000Taxol biosynthesis.]]>205256He, S.L., J.G. Zheng and X.F. Wang et al.,20028558563Ketchum, R.F.B. and D.M Gibson,1996Taxus.]]>46917Passioura, J.B.,2002Review: Environmental biology and crop improvement.]]>29537546Somerville, C.R. and D. Bonetta,2001125168177Shao, H.B.,20012001pp: 123-127pp: 123-127Shao, H.B.,2002124146Shao, H.B., Z.S. Liang, M.A. Shao and B.C. Wang,2005Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism.]]>417377Vasil, I.K.,2002In vitro cell.]]>38383383Wang, W., Q. Shi and P. Zhu et al.,2002Taxus chinensis.]]>44181187Yu, L.J. and C.H. Zhang,1999Taxol biosynthesis promoted by inhibitor.]]>11610Zhao, J. and W.H. Zhu,199829402406Zhou, Z.Q., X.G. Mei and J.L. Chang et al.,2001Taxus chinensis.]]>134243