Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.391.397 (Cola acuminata (Pal. de Beauv, Schott and Endlicher), Cola nitida ((Vent) Schott and Endlicher) and Cola anomala (Schott and Endlicher)) Germplasm in Cameroon]]>OnomoPierre EffaNiemenakNicolas NdoumouDenis Omokolo3200693The aim of this study were to add information on the genetic
structure of Cola sp. and to verify the possibility of classifying Cola
sp. entries based on isoenzyme traits. Polyacrylamide gel electrophoresis
was employed to study the isoenzyme variation of peroxydase, polyphenoloxydase
and amylase in forty nine accessions of three Cameroonian Cola species
(Cola acuminata, Cola nitida and Cola anomala). Band frequencies
were calculated for each entry and for each isoenzyme system. The intrapopulation
variation was estimated by Shannon-Weaver (H)
diversity index. Based on the matrix of band frequencies and standardised data,
the interpopulation variation was examined by cluster analysis. A total of 14
bands with frequency values ranging from 0 to 1 were observed including 12 polymorphic
and 2 monomorphic. The average value of H
estimated for each entry range from 0.07 to 0.63 suggesting that Cola sp.
entries showed a wide polymorphism for all the enzyme systems being tested.
Cluster analysis revealed two distinct groups for both Cola acuminata
and Cola nitida and six for Cola anomala in which entries revealed
a greater similarity.]]>Alba, E., G.B. Polignano, D. De-Carlo and A. Mincione,2001Lathyrus sativus.]]>21520Basaglia, F.,1988Halian ictalunds.]]>89731736Bendiab, K.,1992Phnix dactilifera L.).]]>1992Bhat, K.V., S.R. Bhat and K.P.S. Chandel,1992Musa. II. Peroxidase, superoxide dismutase, shikimate dehydrogenase and malate dehydrogenase.]]>67737743Brown, A.H.D. and R.S. Weir,19831983pp: 219-239pp: 219-239Giovanni, T., R. Alicchio and L. Cinilo,1993Salanum.]]>47237244Hutchenson, K.,197029251254Labdi, M., L.D. Robertson., K.B. Singh and A. Charrier,199688181188Maheswaran, M. and S.R.S. Rangasamy,1992Orysa sativa L.,]]>461520Manzano, A.R., A.A.R. Nodals., M.I.R. Gutierrez., F.Z. Mayor and C.I. Alfonso,2001Colocasia esculenta L. Schott) germplasm in Cuba.]]>1263140McAndrew, B.J. and K.C. Majumber,198330249261Morton, J.F.,1992pp: 739-765pp: 739-765Nkongmeneck, B.A.,19821982Polignano, G.B., G. Quintano, V. Bisignano, P. Uggenti, C.D. Gatta and E. Alba,1998Vicia faba L. minor) accessions. Genetic interpretation and value for classification.]]>102169179Richardson, B.J., P.R. Baverstock and M. Adams,1986pp: 15-218pp: 15-218Rodriguez, M.M., O.M. Parades and V.L. Becerra,2000Lens culinaris Medik).]]>2000pp: 2226Sarangi, N. and A.B. Mandal,1996Labeo rohita (Hamilton).]]>71227230Tanksley, S.D. and C.M. Rick,198057161170