Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.632.635Sacharomyces boulardii in Pathogenesis of Salmonella typhimurium in Experimentally Infected Rats]]>MahzouniehM. I. Karimi T. Zahraei Salehi R. Marjanian 4200694The present research studied the capacity of Saccharomyces boulardii (S. boulardii) to antagonize Salmonella typhimurium (S. typhimurium) in the intestinal tract and humoral response of rats after challenging with S. typhimurium. Sixty conventional male rats were divided at random into 4 groups. The S.boulardii suspensions contained 106, 07 and 108 cells mL-1 were administered (orally) to 3 groups (A, B and C) of rats, respectively for 5 continues days. The rats of group D received Saline instead of S.boulardii and used as control. All of rats challenged with 107 CFU of pathogenic S. typhimurium at fifth day. The viable Salmonella were counted at g-1 of faeces of rates at 0, , 3, 5 and 7 days after challenge. Titer of anti- salmonella antibodies were determined. Present experiments showed that S. boulardii could reduce the bacterial colonization in experimental animals. There was a significant reduction at number of S. typhimurium between control and test groups. A daily dose of 106 yeast cells was resulted in the most reduction in bacterial shedding and high titers of anti salmonella antibodies. The mortality rates were 0 and 46.4% in yeast treatment and control animals, respectively. S. typhimurium viable cells were not detected in the organs of yeast-treated rats. We conclude that S. boulardii has a preventive effect in pathogenesis of S. typhimurium.]]>Andreatti, F.R.L., E. Nepomuceno da Silva, A.R. Ribeiro, N. Kondo and P.R. Curi,2000Salmonella typhimurium and Salmonella entritidis.]]>31107112Agawane, S.B. and P.S. Lonkar,2004Saccharomyces boulardii on experimental ochratoxicosis in broilers: Hematobiochemical studies.]]>5359367Buts, J.P., P. Bernasconi, J.P. Vaerman and C. Dive,1990Saccharomyces boulardii.]]>35251256Corthier, G., R. Lucas, S. Jouvert and F. Castex,1992Saccharomyces boulardii treatment on the activity of Clostridium difficile toxins in mouse digestive tract.]]>3015831589Castagliuolo, I., M.F. Riegler, L. Valenick, J.T. LaMont and C. Pothoulakis,1999Saccharomyces boulardii protease inhibits the effects of Clostridium difficile toxins A and B in human colonic mucosa.]]>67302307Elmer, G.W. and L.V. McFarland,1987Saccharomyces boulardii of toxinogenic Clostridium difficile overgrowth after Vancomycin treatment in hamsters.]]>31129131Fuller, R.,199132439442Filho-Lima, J.V., E.C. Vieira and J.R. Nicoli,2000Lactobacillus acidophilus, Saccharomyces boulardii and Escherichia coli combinations against experimental infections with Shigella flexneri and Salmonella enteritidis subsp. typhimurium in gnotobiotic mice.]]>88365370Santos, Gil de los J.R., O.B. Storch and C. Gil-Turnes,2005Bacillus cereus var. toyoii and S. boulardii increased feed efficiency in broilers infected with Salmonella enteritidis.]]>46494497Laukova, A., P. Guba, R. Nemkova and M. Marekova,2004Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails.]]>494751McFarland, L.V. and P. Bernasconi,1993S. boulardii: A review of an innovative biotherapeutic agent. ]]>6157171Niault, M., F. Thomas, J. Prost, F.H. Ansari and P. Kalfon,1999Saccharomyces species in a patient treated with enteral S. boulardii.]]>28930930Ravdin, J.I., J.E. John, L. Johnston, D.J. Innes and R.L. Guerrant,1985Entamoeba histolytica trophozoites to rat and human colonic mucosa.]]>48292297Rodrigues, A.C.P., R.M. Nardi, E.A. Bambirra, E.C. Vieira and J.R. Nicoli,1996Saccharomyces boulardii against experimenttal oral infection with Salmonella typhimurium and Shigella flexneri in conventional and gnotobiotic mice.]]>81251256Roffe, C.,199632110Zbinden, R., E.E. Gonczi and M. Altwegg,1999Saccharomyces boulardii (nom. inval.) on cell invasion of Salmonella typhimurium and Yersinia enterocolitica.]]>11158162