Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1452.1458Haemonchus longistipes Purified Antigens in Diagnosis of Gastro-intestinal Nematodes Infection in Camels]]>El-BahyM.M. El-BahyN.M. ShalabyH.A. 92007109Fractionated Haemonchus longistipes crude antigen revealed 7 protein fractions at molecular weights of 93, 74, 67, 56, 32, 26 and 16 kDa. The bands at 56, 32 and 26 kDa reacted specifically with H. longistipes hyper-immune sera prepared in rabbit (1:100 dilution) using EITB technique. The eluted concentrated fractions as well as crude worm antigens were used in diagnosis of natural infection of Gastro-Intestinal Nematodes (GIN) only or associated with other parasites in camels using ELISA technique. H. longistipes crude antigen and fraction of 26 kDa induced high sensitivity in diagnosis of infection in animals harboring GIN only (sensitivity was 95-100%), while it was 85.0-93.3% with 32 kDa fraction and 55.5-73.3% with that of 56 kDa using ELISA technique. The two H. longistipes eluted protein fractions of 26 and 32 kDa showed higher sensitivity than their crude antigens in diagnosis of GIN infection mixed with other parasites. While, Protein bands of 26 kDa appeared more sensitive than the other protein bands in detection of anti- H. longistipes antibodies at higher serum dilution. With special superiority for fraction of 26 kDa, in comparison with the other antigens, sharp specificity or sensitivity in diagnosis, could not be achieved in this study especially in case of mixed infection with other parasites, where these animals might be immune-compromised.]]>Abdel-Rahman, S.M., K.L. OReilly and J.B. Malone,1998F. hepatica coproantigen in cattle.]]>59533537Adams, D.B. and K.J. Beh, 1981Haemonchus contortus.]]>11381386Bekele, T.,2002Camelus dromedarius) in semi-arid lands of eastern Ethiopia.]]>105139152Burger, H.J. and M. Stoye,19683122Charley-Poulain, J., G. Luffau and P. Pery,1984Haemonchus contortus.]]>14129141Christensen, C.M., D.S. Zarlenga and L.C. Gasbarre,19947893100De Morilla, A.C., A. Gomez, C.R. Bautista and A. Morilla,1983Fasciola hepatica en diferentes pruebas immunologicas par el diagnostico de la fascioliasis en bovinose.]]>444145Espino, M., B.E. Dumenigo, R. Fernandez and C.M. Finaly,198737605608Fetterer, R.H.,1989Haemonchus contortus--I. Isolation and partial characterization.]]>94383388Ford, M.J., M.E. Taylor and Q.D. Bickle,1987F. hepatica antigens for immunization against S. mansoni infection.]]>9427336Gomez-Munoz, M.T., M. Cuquerella and J.M. Alunda,1996Haemonchus contortus.]]>26311318Gomez-Munoz, M.T., I.A. Dominguez, L.A. Gomez-Iglesias, F.J. Fernandez-Perez and S. Mendez et al.,200012354360Haig, D.M., R. Windon, W. Blackie, D. Brown and W.D. Smith,1989Haemonchus contortus.]]>11463477Ibarra, F., N. Montenegro, Y. Vera, C. Boulard, J. Flore and P. Ochoa,199877229236Jacquiet, P., J. Cabaret, M.L. Dia, D. Cheikh and E. Thiam,1996Haemonchus longistipes in dromedaries of Saharo-Sahelian areas of Mauritania.]]>66193204Katrak, K., B.P. Mahon, W.C. Jones, S. Brautigam and K.H. Mills,1992in vitro.]]>156247254Khalil, H.M., T.M. Abdel-Aal, M.K. Makhaled, H.M. Abdallah, I.A. Fahmy and E.A. El-Zayyat,1990Fasciola antigens in immunodiagnosis of human fascioliasis.]]>208794Laemmli, U.K.,1970227680685Langley, R.J. and G.V. Hillyer,198941472478Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Petit, A., P. Pery and G. Luffau,19811219Rathore, D.K., S. Suchitra, M. Saini, B.P. Singh and P. Jashi,2006Haemonchus contortus excretory/secretory antigen that inhibits host monocytes.]]>138291300River Marrero, C.A., N. Santiago and G.V. Hillyer,1988Fasciola hepatica.]]>74646652Schallig, H.D.F.H., M.A.W. Van Leeuwen and W.M.L. Hendrikx,1994Haemonchus contortus.]]>108351357Smith, S.K. and W.D. Smith,1996Haemonchus contortus and with its major polypeptide components.]]>6016Soulsby, E.J.,19867th Edn.,pp: 212-242pp: 212-242Towbin, H., T. Staehelin and J. Gordon,19797643504354Tsai, C.M. and C.E. Frasch,1982119115119Welch, R.D., P.H. Smith, J.B. Malone, R.A. Holmes and J.P. Geaghan,1987Fasciola hepatica infection in Louisiana cattle by an ELISA.]]>48345347Yamaguti, S.,1959