Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.193.195Toxoplasma gondii from Sera and Urine of Experimentally Infected Mice by PCR]]>ShojaeeSaeedeh RezaieSasan KeshavarzHossein 12007101Toxoplasms gondii was detected in sera and urine of acutely infected mice. Animals were inoculated intraperitoneally with tachyzoites of T. gondii, RH strain. Ten animals were killed every day from day 1 up to day 7 post infection. Urine and sera of animals were collected and stored at -20°C until use. PCR performed by B1 gene amplification. Toxoplasma was detected in sera from 3 days post infection and in urine from 5 days post infection. No parasite was detected in control group.]]>Angel, S.O., M. Matrajt, J. Margarit, M. Nigro and E. Illescas et al.,199735591595Burg, J.L., C.M. Grover, P. Pouletty and J.C. Boothroyd,1989Toxoplasma gondii, by polymerase chain reaction.]]>2717871792Fuentes, I., M. Rodriguz., C.J. Domingo, F. Castillo, T. Juncosa and J. Alvar,19963423682371Grover, C.M., P. Thulliez, J.S. Remington and J.C. Boothryd,19902822972301Hafid, J., R. Tran Manh Sung and B. Posseto,1991274045Hafid, J., R. Tran Manh Sung, H. Raberin, Z.Y. Akono, B. Pozzetto and M. Jana,1995Toxoplasma gondii in human infection.]]>52336339Hafid, J., D. Guichard, P. Flori, T. Bourlet, H. Raberin, C. Genin and R.T. Sung,2000Toxoplasma gondii by polymerase chain reaction in sera of acutely infection mice.]]>86857859Ho-Yen, D.O., A.W. Joss, A.H. Balfour, E.T.M. Smyth, D. Baird and J.M.W. Chatterton,1992Toxoplasma gondii in human blood samples.]]>45910913Huskinson, J., P. Stepick-Biek and J.S. Remington,19892710991101Johnson, J.D., P.D. Butcher, D. Savva and R.E. Holliman,199326147158Joss, A.W.L., J.M.W. Chatterton, R. Evans and D.O. Ho-Yen,1993Toxoplasma polymerase chain reaction of experimental blood samples.]]>383843Nguyen, T.D., M. DE Kesel, G. Bigaigenon, P. Hoet, G. Pazzaglia, M. Lammens and M. Delmee,1996Toxoplasma gondii tachyzoites and bradyzoites in blood, urine and brains of infected mice.]]>3635639Raizman, R.E. and F.A. Neva,1975Toxoplasma gondiij.]]>1324448Savva, D., J.C. Morris, J.D. Johnson and R.E. Holliman,1990Toxoplasma gondii.]]>322531Van Knapen, F. and S.O. Panggabean,1977Toxoplasma gondii by enzyme-linked immunosorbent assay.]]>6546547Wiess, L.M., S.A. Udem, M. Salgo, H.B. Tanowitz and M. Wittner,1991Toxoplasma DNA in an experimental murine model: Use of Toxoplasma gondii specific cDNA and the polymeras chain reaction.]]>163180186Weiss, J.B.,19958113130