Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2517.2521In vitro Propagation of Lilium longiflorum Var. Ceb-Dazzle Through Direct Somatic Embryogenesis]]> khosraviSolmaz AzghandiAli Vatanpour MojtahediNarges HaddadRaheem 1220071015Experiments were carried out to investigate the effects of various concentrations of Picloram (0, 1, 2, 3, 6 and 9 mg L-1), TDZ (0, 0.5, 1, 1.5 and 2 mg L-1), NAA (1.5 mg L-1) in combination with TDZ (0.08, 0.2 and 0.4 mg L-1), 2,4-D (2.5, 5 and 10 mg L-1) combined with BAP (0.25 mg L-1) and different types of explants (basal, central and distal part of the bulb scale) on direct somatic embryogenesis induction of Lilium longiflorum var. Ceb-Dazzle. The explants were surface sterilized and cultured on MS medium supplemented with 3% sucrose, 0.3% Phytagel and various concentrations of mentioned growth regulators. It was found that Picloram at a concentration of 2 mg L-1 was the most effective treatment for induction of direct somatic embryogenesis and gave the highest number of embryos (18.6) on each explant. The explants from basal part of the bulb scale showed the best responses (19.9 embryos/explant). TDZ alone or combined with NAA in various concentrations was not able to induce somatic embryogenesis, but gave direct bulblet regeneration. Similar results were obtained for 2, 4-D and BAP combination treatments. Induced somatic embryos were transferred to MS medium without growth regulators for maturation and matured plantlets were successfully acclimatized and transferred to in vivo conditions.]]>Agricultural Statistics,2004Bach, A.,1992Freesia hybrida.]]>325429434Bashirazami, A.,19961996Duncan, D.B.,195511142Gude, H. and M.H.G.E. Dijkema,1997430275280Jeong, J.H.,1996In vitro propagation of bulb scale section of several Korean native lilies.]]>414749760Karun, A., E.A. Siril, E. Radha and V.A. Parthasarathi,2004Areca cathecu L.).]]>8616231628Kim, S.K., J.S. Lee, K.H. Haung and B.J. Ahn,2003625253259Maesato, K., K. Sharada, T. Hara and K.S. Sarma,1994In vitro bulblet regeneration from bulb scale explants of Lilium japonicum Thunb: Effect of plant growth regulators and culture environment.]]>69289297Merkle, S.A., W.A. Parrot and B.S. Flinn,19951995pp: 155-203pp: 155-203Murashige, T. and F. Skoog,196215473497Nakano, M., T. Sakakibara, S. Suzuki and H. Saito,2000Lilium longiflorum Wallace and restoration by the auxin transport inhibitor 2, 3, 5,-triiodobenzoic acid.]]>158129137Nuth, D.T., V.B. Le, J.A. Teixeira De Silva and C.R. Aswath,2001Lilium: Regeneration and transformation perspectives.]]>37516523Nuth, D.T., V.B. Le and K.T. Thanh,2001Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin.]]>374449Nuth, D.T., V.B. Le, T.N. Minh, J. Teixeira De Silva, S. Fukai, M. Tanaka and K.T.T. Van,2002Lilium longiflorum.]]>37193198Suzuki, S., Y. Nimi, T. Sakakibara, K. Hosokawa, S. Yamamura and M. Nakano,1998Lilium formasanum calli and transient expression of the guse. A gene after co-cultivation with Agrobacterium tumefaciaens.]]>15213216Tribulato, A., P.C. Remotti and H.J.M. Loffler,1997Lilium longiflorum.]]>447205206Varshney, A., V. Dhan and P.S. Sirivastava,2000in vitro mass propagation of lily through liquid stationary culture.]]>36383391