Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2856.2861Taxus cuspidata Var. nana]]>TachinbanaSanro MuranakaToshio ItohKazutaka 1220071017To stimulate the production of taxol (paclitaxel) by cell suspension cultures of Taxus cuspidata var. nana, two kinds of elicitors and a biogenetic precursor were used in F4G4 culture medium. Paclitaxel production was enhanced by each elicitor as well as by the biogenetic precursor and by a combination of the two elicitors or one elicitor and the biogenetic precursor. The amount of paclitaxel produced (16.6 mg L-1) was greatest when the cell suspension cultures were conducted in F4G4 medium containing chito-heptaose (8 mg L-1) and jasmonic acid (21 mg L-1). The productivity was enhanced 4.1 fold compared to the control. The amount of paclitaxel produced was increased by supplying air to the cultures, though the productivity depended on the amount of air supplied. However, no enhancement of production was observed when a combination of air and chito-heptaose was provided to the cell suspension cultures.]]>Baebler, Š., M. Camloh, K. Maja, M. Ravnilar and L. ðel,2002TaxusHmedia).]]>68475476Choi, H.K., S.I. Kim, J.S. Son, S.S. Hong, H.S. Lee, I.S. Chung and H.J. Lee,2000Taxus chinensis cells.]]>2217931796Fett-Neto, A.G., S.J. Melanson, S.A. Nicholson, J.J. Pennington and F. Di Cosmo,1994Taxus cuspidata.]]>44967971Fett-Neto, A.G., W.Y. Zhang and F. DiCosmo,1994Taxus cuspidata.]]>44205210Fleming, P.E., A.R. Knaggs, X.G. He, U. Mocek and H.Z. Floss,199411641374138Furmanowa, M., H. Oledzka, K. Syklowska-Baranek, J. Jóefowicz and S. Gieracka,2000Taxus cuspidata and Taxus H media by some elicitors and precursors.]]>2214491452Guo, D.M., Y.C. Liu and C.S. Chen,19935812871289Ketchum, R.E.B., D.M. Gibson, R.B. Croteau and M.L. Shuler,19996297105Khosroushahi, A.Y., M. Valizadeh, A. Ghasempour, M. Khosrowshahi, H. Naghdibadi, N.R. Dapour and Y. Omidi,2006Taxus baccata.]]>30262269Kim, B.J., D.M. Gibson and M.L. Shuler,2005Taxus sp. suspension cultures elicited with methyl jasmonate.]]>21700707Kingston, D.G.I., A.G. Chaudhary, A.A. Gunatilaka and M.L. Middleton,19943544834484Linden, J.C. and M. Phisalaphong,2000Taxus canadensis.]]>1584151Mirjalili, N. and J.C. Linden,1996Taxus cuspidaa: Ethylene interaction and induction models.]]>12110118Muranaka, T., M. Yoshida, K. Itoh andS. Tachibana,2004Taxus cuspidate variety nana.]]>7399405Navia-Osorio, A., H. Garden, R.M. Cusidó, J. Palazón, A.W. Alfermann and M.T. Piñol,2002Taxus baccata and Taxus wallichiana in an airlift bioreactor.]]>15997102Ojima, I., R. Geney, I.M. Ungureanu and D. Li,200253269274Szabo, E., A. Thelen and M. Petersen,1999Coleus blumei.]]>18485489Tachibana, S., E. Watanabe, J. Ueno, K. Tokubuchi and K. Itoh,2005Taxus cuspidata var. nana.]]>51176180Tachibana, S., A. Matsuo, K. Itoh and T. Oki,1994Taxus cuspidata Sieb and Zucc. var. nana Rehder.]]>4010081013Tanaka, H.,19921992Wang, C., J. Wu and X. Mei,2001Taxus chinensis cell culture by fungal elicitation and medium renewal.]]>55404410Wani, M.C., H.L. Taylor, M.E. Wall, P. Coggon and A.T. Mcphail,1971Taxus brevifolia. ]]>9323252327Wang, Y.D., Y.J. Yuan and J.C. Wu,2004Taxus chinensis var. mairei.]]>19259265Yoshida, M., M. Toshio, I. Kazutaka and T. Sanro,2002Taxus cuspidate variety nana callus cultures.]]>5461465Yuan, Y.J., C. Li, Z.D. Hu and J.C. Wu,2001Taxus chinensis var. mairei induced by oligosaccharides from Fusarium oxysprumM.]]>29372379Yukumune, Y., H. Tabata, Y. Higashi and Y. Hara,1996Taxus cell suspension cultures.]]>1411291132Zhang, C.H., X.G. Mei, L. Liu and L.J. Yu,2000Taxus chinensis.]]>2215011504Zhang, C.H. and H.B. Xu, 2001Taxus chinensis.]]>23189193Zhang, C.H., P.S. Fevereiro, G. He and Z. Chen,2007Taxus chinensis cell suspension cultures adapted to chitosan.]]>172158163