Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3141.3145Agrobacterium Transformation System on Transient GUS Expression in Hypocotyls of Rapeseed (Brassica napus L. cv. PF704) Microspore-Derived Embryos]]>AbdollahiM. R.MoieniA. MousaviA. SalmanianA. H.JavaranM. JalaliMajdiM. 1220071018A new method for transformation of rapeseed microspore-derived embryos (MDEs), based on microwounding of MDEs by particle bombardment prior to inoculation with an Agrobacterium suspension was reported. In this study, effects of two transformation systems (integrated bombardment and Agrobacterium transformation system and the singular bombardment) on transient GUS expression in hypocotyls of rapeseed (Brassica napus L. cv. PF704) MDEs were studied. Bombardment parameters were: helium pressure, 1350 psi; distance between stopping screen and target tissue, 9 cm, gold particles size of 1.0 μm and chamber vacuum pressure, 24 in Hg. In integrated system A. tumefaciens strain AGL1 carrying the binary vector pCAMBIA3301 was used. Bombarded hypocotyls of MDEs were inoculated with Agrobacteria at OD600 = 1.0 for 10 min or OD600 = 0.25 for 24 h. Integrated transformation system increased the mean number of blue spots about 2-2.5 fold compared to singular bombardment and inoculated hypocotyls with OD600 = 1.0 for 10 min produced the highest number of blue spots per bombardment (743±23.67).]]>Bidney, D., C. Scelonge, J. Martich, M. Burrus, L. Sims and G. Huffman,1992Agrobacterium tumefaciens.]]>18301313Brasileiro, A.C.M., F.J.L. Aragão, S. Rossi, D.M.A. Dusi, L.M.G. Barros and E. Rech,1996Agrobacterium sp. strains and improvement of Agrobacterium-mediated transformation using microprojectile bombardment.]]>121810815Chen, J.L. and W.D. Beversdorf,199488187192Curtis, I.S. and H.G. Nam,2001Raphanus sativus L. longipinnat us Bailey) by floral-dip method-plant development and surfactant are important in optimizing transformation efficiency.]]>10363371Droste, A., G. Pasquali, M. Helena and B. Zanettini,2000Agrobacterium transformation system: An alternative method for soybean transformation.]]>185159Fletcher, R., J. Coventry and L.S. Kott,1998Brassica napus, Technical Bulletin.]]>Fukuoka, H., T. Ogawa, M. Matsuoka, Y. Ohkawa and H. Yano,1998Brassica napus L.) and the production of fertile transgenic plants.]]>17323328Gamborg, O.L., R.A. Miller and K. Ojima,196850151158Jefferson, R.A.,19875387405Keller, W.A., Z. Fan, P. Pechan, N. Long and J. Grainger,1987Brassica napus.]]>1987pp: 152157Khush, G.S. and S.S. Virmani,19961996pp: 11-34pp: 11-34Klein, T.M., E.C. Harper, Z. Svab, J.C. Sanford, M.E. Fromm and P. Maliga,1988Nicotiana cells by the particle bombardment process.]]>8585028505Kosugi, S., Y. Ohashi, K. Nakajima and Y. Arai,199070133140Lazo, G.R., P.A. Stein and R.A. Ludwig,1991Arabidopsis genomic library in Agrobacterium.]]>9963967Lichter, R.,1982Brassicacea species.]]>103119123Loh, C.S. and D.S. Ingram,1982Brassica napus sp. oleifera.]]>95359366May, G.D., R. Afza, H.S. Mason, A. Wiecko, F.J. Novak and C.J. Arntzen,1995Musa acuminata) plants via Agrobacterium-mediated transformation.]]>13486492Nehlin, L., C. Mollers and K. Glimelius,1995Brassica napus L.]]>111219227Nehlin, L., C. Mollers, P. Bergman and K. Glimelius,2000Brassica napus L.]]>156175183Sangwan, R.S., C. Ducrocq and B.S. Sangwan-Norreel,1993Datura innoxia and Nicotiana tabacum: Production of transgenic haploid and fertile homozygous dihaploid plants.]]>9599115Sangwan, R.S., F. Dubois, C. Ducrocq, Y, Bourgeois, B. Vilcot, N. Pawlicki and B.S. Sangwan-Norreel,19951995pp: 271277