Pakistan Journal of Biological Sciences
1028-8880
1812-5735
Asian Network for Scientific Information
10.3923/pjbs.2008.1360.1364
Orozco Benitez
Maria Guadalupe
Lemus Flores
Clemente
Hernandez Ballesteros
Juan Antonio
Navarrete Mendez
Raul
Juarez-Mosqueda
Maria de Lourdes
10
2008
11
10
Samples of semen from 12 pigs, three from Yorkshire,
Landrace, Duroc and Mexican Hairless each where obtained to study cryopreservation
methods. Three stages of boar semen cryopreservation were evaluated: none
(fresh stage), cooling at 5 °C and freezing at -196 °C then thawing
to 56 °C for 12 sec. Perinuclear theca damage and domain alterations
were selected as indices of seminal quality, as measured by electronic
and fluorescence microcopy, respectively according to two lineal models
considering by separately the effect of semen preservation and breed.
Integrity and absence of perinuclear theca significantly (p<0.001)
decreased and increased, respectively according to a decrease in temperature
of cryopreservation, from 87.4 to 58.8% and from 0.8 to 26.2%, respectively.
This same significant (p<0.001) effect was found for acrosomal and
post-acrosomal membrane distribution of domains, from 92.1 to 76.8% and
from 3.1 to 13.1% in this same order. Slight but highly significant (p<0.001)
differences were observed when theca integrity was evaluated as affected
by breed, with highest and lowest values for Yorkshire and Pelón
Mexicano pigs, respectively. No breed effect was encountered for presence
of acrosomal domains. A strong interdependence was found between perinuclear
theca damage and domain distribution. In this connection, a highly significant
(p<0.001) positive, interdependence was observed between the theca
damage and acrosomal domain (r = 0.87), while this same relationship was
although highly significant (p<0.001), negative in nature for equatorial
and post-acrosonal domains (r = -0.77 and -0.85, respectively). This experiment
confirmed that cryopreservation methods may severely affect semen quality
of pigs and that genotype may further influence these same indices. More
research is needed for improving methods of preservation of pig semen
quality, from the point of view of perinuclear theca and domain characteristics
of spermatozoa.]]>
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