Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1541.1550Triticum aestivum (cv.
Baccross and Mahdavi) to the Dual Inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D]]>MehryAskary AkbarMostajeran GitiEmtiazi 1220081112The potential enhancement of root colonization and nitrogenase
activity of wheat cultivars (Baccross and Mahdavi) was studied with application
of two Azospirillumbrasilense strains (native and Sp7)
co-inoculated with two Rhizobium meliloti strains (native and DSMZ
30135). The results indicated that the colonization was different due
to the strains and cultivars of wheat were used. Native A. brasilense
colonized wheat root better than Sp7 strain. However, Baccross cv. reacted
better with native Azospirillum compared to Mahdavi cv. which reacted
better with Sp7. When plants inoculated with dual inoculants (SP7 with
standard Rhizobium), the colonization of Azospirillum were
increased significantly (from 1.67x105
to 22x105 cfu g-1
FW for Baccras cv. and 3.67x105
to 26x105 cfu g-1
FW for Mahdavi cultivar). Whenthe standard Rhizobium as
co-inoculants changed to the native Rhizobium, the colonization
of Azospirillum was higher when compared to the single inoculants
but was almost the same when compared to the standard Rhizobium.When the standard or native strains of Rhizobium used as single
inoculation of wheat roots, the number of Rhizobium in the wheat
roots were not changed significantly. However, when plants co-inoculated
with Rhizobium and Azospirillum, the colonization of Rhizobium
was increased. Co-inoculation of standard strain of R. melilot with
A. brasilense Sp7 showed that the colonization of Rhizobium
were increased from 0.67x105
to 21x105 cfu g-1
FW for Baccross cv. and 0.33x105
to 18x105 cfu g-1
FW for Mahdavi cv. This behavior was the same when inoculation of Rhizobium
was happened with the native one. In dual inoculation, the highest nitrogenase
activity was measured in combination of the local strains (native A.
brasilense with the native R. meliloti)and the lower
one belongs to the combination of standard strains (Sp7 with standard
R. meliloti). The difference in nirtogenase activity for different
cultivars of wheat with Sp7 and standard Rhizobium is not significant
but the difference for Sp7 strain plus native Rhizobium is significant
(p>0.05). However, the differences were not significant (p<0.05)
for nitrogenase activity in bacterial tubes, the difference for nitrogenase
activity of co-inoculated plants with combination of Sp7 and Rhizobium
either standard or native were significantly different ]]>Al-Mallah, M.K., M.R. Davey and E.C. Cocking,1990Brassica napus).]]>4115671572Amooaghaie, R., A. Mostajeran and G. Emtiazi,2002Azospirillum brasilense strains on proton efflux of intact wheat roots.]]>243155160Antoun, H., C.J. Beachamp, N. Goussard, R. Chabot and R. Lalande,1998Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: Effects on radishes (Rhaphanus sativus L.).]]>2045667Baldani, I.J. and L.V. Baldani,200577549579Bashan, Y. and H. Levanony,1985Azospirillum brasilense.]]>31947952Bashan, Y. and H. Levanony,1989119191197Bashan, Y. and G. Holguin,1997Azospirillum-plant relationships: Environmental and physiological advances (1990-1996).]]>43103121Bashan, Y., G. Holguin and L.E. de-Bashan,2004Azospirillum-plant relationship: Physiological, molecular, agricultural and environmental advances (1997-2003).]]>50521577Bashan, Y. and L.E. de-Bashan,20052005pp: 103-115pp: 103-115Biro, B., K. Koves-Pechy, I. Voros, T. Takacs, P. Eggenberger and R.J. Strasser,2000Azospirillum and Rhizobium nitrogen-fixers and arbuscular mycorrhizal fungi in the rhizosphere of alfalfa in sterile, AMF-free or normal soil conditions.]]>15159168Christiansen-Weniger, C.,1997Azospirillum brasilense C3: gusA inhabiting induced tumors along stem and roots of rice.]]>29943950Chirstiansen-Weniger, C.,1998175576Cocking, E.C., M.R. Davey, S.L. Kothari, J.S. Srivastava, Y. Jing, R.W. Ridge and B.G. Rolfe,199314123130Cocking, E.C., C.A. Batchelor, S.L. Kothari, S. Jain, G. Webster, J. Jones, J. Jotham and M.R. Davey,199486772Cocking, E.C.,2005in vitro.]]>41369373Cocking, E.C., P.J. Stone and M.R. Davey,200548888896Dalla Santa, O.R., R.F. Hernandez, G.L.M. Alvarez, P. Ronzelli Junior and C.R. Soccol,2004Azospirillum sp. inoculation in wheat, barley and oats seeds greenhouse experiments.]]>47843850Denison, R.F.,2000156567576Denison, R.F. and E.T. Kiers,2004612351239Dobbelaere, S., A. Croonenboghs, A. Thys, D. Ptacek and J. Vanderleyden et al.,2001Azospirillum.]]>28871879Elanchezhian, R. and J.D.S. Panwar,1997Azospirillum brasilense on nitrogen fixation, photosynthesis and grain yield in wheat.]]>178129133Er, F., M. Kilic, A.R. Brohi and Ogut,2004Rhizobium etli strains and Azospirillum brasilense.]]>48124133Fages, J. and J.F. Arsac,1991Azospirillum and other plant growth promoting rhizobacteria.]]>1378790Fahraeus, G.,195716374381Favilli, F., R. Pastorelli and A. Gori,1993Azospirillum bacterization in field experiments.]]>123281285Gough, C., J. Vasse, C. Galera, G. Webster, E. Cocking and J. Denarie,1997Arabidopsis thaliana as a model plant.]]>194123130Hardy, R.W.F., R.D. Holsten, E.K. Jackson and R.C. Burns,19682 fixation: Laboratory and field evaluation.]]>4311851207Hardy, R.W.F., R.C. Burns and R.D. Holsten,197354781Hirsch, A.M., M.R. Lum and D.J. Allan Downie,200112714841492Hoflich, G., W. Wiehe and G. Kuhn,199450897905Hoflich, G.,1999Rhizobium bacteria. ]]>1999Holguin, G. and Y. Bashan,1996Azospirillum brasilense Cd is promoted when co-cultured with a mangrove rhizosphere bacterium (Staphylococcus sp.).]]>2816511660Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley and S.T. Williams,19949th Edn.,Pages: 787Pages: 787Humphry, R.D., M. Andrews, R.S. Santos, K.E. James, V.L. Vinogradova, L. Perin, M.V. Reis and P.S. Cummings,2007Rhizobium radiobacter strain used as a biofertiliser on graminaceous crops in Russia.]]>91105113Itzigsohn, R., Y. Kapulnik, Y. Okon and A. Dovrat,1993Rhizobium meliloti and alfalfa (Medicago sativa) in association with Azospirillum brasilense.]]>39610615Knowles, R.,19821982pp: 1071-1092pp: 1071-1092Kucey, R.M.N.,198834735739Mateos, P.F., J.I. Jimenez-Zurdo, L. Chen, A.S. Squartini and S.K. Haack et al.,1992Rhizobium leguminosarum biovar trifolii.]]>5818161822Matiru, V.N. and F.D. Dakora,2004317McInroy, J.A. and J.W. Kloepper,1995173337342Molla, A.H., M.S. Shamsuddin, M. Morziah and A.B. Puteh,2001Azospirillum and Bradyrhizobium in laboratory systems.]]>33457463Mostajeran, A., R. Amooaghaie and G. Emtiazi,2007Azospirillum brasilense on wheat roots.]]>291239248Ogut, M., C. Akdag, O. Duzdemir and M.A. Sakin,2005Azospirillum/Trichoderma: The effects on dry bean and wheat.]]>41262272Reinhold-Hurek, B., T. Hureck, M. Claeyssens and M. Van-Montasu,1993Escherchia coli and characterization of cellulolytic enzyrnes of Azoarcus sp.]]>17570567065Rodelas, B., J. Gonzalez-Lopez, M.V. Martinez-Toledo, C. Pozo and V. Salmeron,1999Rhizobium/Azotobacter and Rhizobium/Azospirillum combined inoculation on mineral composition of faba bean (Vicia faba L.).]]>29165169Rueda-Puente, E.O., T. Castellanos, E. Troyo-Dieguez and J.L. Diaz-d-Leon-Alvarez,2004Klebsiella pneumoniae and Azospirillum halopraeferens on the growth and development of two Salicornia bigelovii genotypes.]]>446574Sadovnikova, Y.N., L.A. Bespalova and L.P. Antonyuk,2003Azospirillum brasilense.]]>389103105Saubidet, M.I., N. Fatta and A.J. Barneix,2002Azospirillum brasilense on growth and nitrogen utilization by wheat plants.]]>245215222Shaban, G.M., E.M. Fadl-Allah and H.M. El-Komy,1997Azospirillum-maize association.]]>32309327Spencer, D., E.K. James, G.J. Ellis, J.E. Shaw and J.I. Sprent,19944514751482Thuler, D.S., E.I.S. Floh, W. Handro and H.R. Barbosa,2003Azospirillum sp. in chemically defined media.]]>37174178Tripathi, A.K. and B.M. Mishra,1996Azospirillum lipoferum locus that complements Rhizobium melilotidctA and dctB mutations.]]>42503506Tsavkelova, E.A., S.Y. Klimova, T.A. Cherdyntseva and A.I. Netrusov,200642117126Van Rhijin, P. and J. Vanderleyden,1995Rhizobium-plant symbiosis.]]>59124142Vincent, J.M.,19701970pp: 1-13pp: 1-13Webster, G., V. Jain, M.R. Davey, C. Gough, J. Vasse, J. Denarie and E.C. Cocking,1998Azorhizobium caulinodans.]]>21373383Yanni, Y.G., R.Y. Rizk, V. Corich, A. Squartini and K. Ninke et al.,1997Rhizobium leguminosarum bv. trifolii and rice roots and assessment of its potential to promote rice growth.]]>19499114Zaady, E., A. Perevolotsky and Y. Okon,1993Azospirillum brasilense Cd.]]>25819823Zeman, A.M.M., Y.T. Tchan, C. Elmerich and I.R. Kennedy,1992Azospirillum.]]>143847855Zimmer, W. and H. Bothe,1988Azospirillum and wheat.]]>110239247