Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2193.2200C. acetobutylicum]]>AlshiyabH. KalilM.S. HamidA.A. YusoffW.M. Wan 1220081118The objective of this study is to investigate the effect
of salts addition to fermentation medium on hydrogen production, under
anaerobic batch culture system. In this study, batch experiments were
conducted to investigate the inhibitory effect of both NaCl and sodium
acetate on hydrogen production. The optimum pH and temperature for hydrogen
production were at initial pH of 7.0 and 30°C. Enhanced production
of hydrogen, using glucose as substrate was achieved. In the absence of
Sodium Chloride and Sodium Acetate enhanced hydrogen yield (YP/S)
from 350 mL g-1 glucose utilized to 391 mL g-1 glucose
utilized with maximum hydrogen productivity of 77.5 ml/L/h. Results also
show that sodium chloride and sodium acetate in the medium adversely affect
growth. Hydrogen yield per biomass (YP/X) of 254 ml/L/g, biomass
per substrate utilized (YX/S) of 0.268 and (YH2/S)
of 0.0349. The results suggested that Sodium at any concentration resulted
to inhibit the bacterial productivity of hydrogen.]]>Ahring, B.K. and P. Westermann,198317365370Bechtle, R.M.,19695217331737Biggins, D.R. and M.J. Dilworth,1968Clostridium pasteurianum by ADP and acetyl phosphate.]]>156285296Booth, I.R.,198549359378Chia-Jenn, W., D. Robert, D. Tanner, W. George and M. Malaney,198243757763Das, D. and T.N. Veziroglu,2001261328Donnison, A.M., C.M. Brockelsby, H.W. Morgan and R.M. Daniel,19893314951499Evvyernie, D., K. Morimoto, S. Karita, T. Kimura and K. Sakka,200191339343Joliffe, L.K., R.J. Doyle and U.N. Streips,198125753763Kataoka, N., A. Miya and K. Kiriyama,1997364147Kemper, M.A., M.M. Urrutia, T.J. Beveridge, A.L. Koch and R.J. Doyle,1993Bacillus subtilis .]]>17556905696Lawrence, R.C. and W.B. Sanderson,1969362121Lee, Y., T. Miyahara and T. Noike,200180227231Lin, C.Y. and C.H. Lay,200530285292Maris, G., N. Hartmanis and G. Sten,1984Clostridium acetobutylicum: Levels of enzymes involved in the formation of acetate and butyrate.]]>4712771283Miller, G.L.,195931426428Mizuno, O., R. Dinsdale, F.R. Hawkes, D.L. Hawkes and T. Noike,2000735965Morimoto, M., M. Atsuko, A.A.Y. Atif, M.A. Ngan and A. Fakhrul-Razi et al.,200429709713Nandi, R. and S. Sengupta,1998246184Niel, E.W.J., P.A.M. Claassen and A.J.M. Stam,2003Caldicellulosiruptor saccharolyticus.]]>81255262Saint-Amans, S., L. Girbal, J. Andrade, K. Ahrens and P. Soucaille,200118317481754Taguchi, F., N. Mizukami, T. Saito-Taki and K. Hasegawa,1995Clostridium sp. strain No. 2. ]]>41536540Van Andel, J.G., G.R. Zoutberg, P.M. Crabbendam and A.M. Breure,1985Clostridium butyricum grown under a self generated gas atmosphere in chemostat culture.]]>232126Wang, G. and D. Wang,1984Clostridium thermoaceticum .]]>47294298Wooshin, P., H.H. Seung, O.H. Sang-Eun, B.E. Logan and I.S. Kim,20052 increases biological hydrogen production.]]>3944164420Xiaolong, H., Z. Minghua, Y. Hanqing, S. Qinqin and Lei Lecheng,200614511517Zhang, H.B., A. Mary and E.L. Bruce,2006Clostridium acetobutylicum in an unsaturated flow reactor.]]>40728734Zheng, X.J., Y.M. Zheng and H.Q. Yu,20052610731080