Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2472.2477BALB/c Mice after Immunization with Brucella Ribosomal Gene]]>AbtahiH. SalmanianA.H. RafatiS. NejadG.B. SaffariM. GhazaviA. MosayebiG. 1220081121This study was evaluated the ability of DNA vaccine
encoding L7/L12 protein of Brucella sp. to induce cellular and
humoral immune responses in BALB/c mice and the profile of cytokines
and IgG sub classes were determined. Intra muscular vaccination of mice
using L7/L12 gene. Three vaccinations at 3 week intervals were performed.
Cytokines and IgG subclasses were analyzed 3 week after the last DNA vaccination.
Splenic lymphocytes from L7/L12pCDNA3-vaccinated mice produced high levels
of IFNγ (3100 pg mL-1) and low levels of IL-5 (300 pg
mL-1), 3 weeks post-vaccination. The L7/L12pCDNA3 immunizations
elicited high IgG2a isotype response in mice immunized. This antigen also
induced IgG1 titers which were slightly lower than the IgG2a titers. Immunological
analysis shows the appropriate immune response in BALB/c mice model
after vaccination with L7/L12 gene. The high level of IFNγ and low
level of IL-5 in combination with high IgG2a/ IgG1 ratio show the activation
of Th1 cell response. The lower bacterial cfu from vaccinated mice in
comparison with control groups show the efficiency of L7/L12 DNA vaccination
in mice model.]]>Abtahi, H., A.H. Salmanian, S. Rafati, G. Behzadian Nejad and Z.M. Hassan,2004Brucella abortus and its reactivity with infected human sera.]]>81318Adone, R. and F. Ciuchini,2001Brucella abortus RB51 and hot saline extract from Brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with Brucella abortus RB51.]]>8119122Aleixo, M.J., M.L. Ferreira and F. Antunes,199912323330Boschiroli, M.L., V. Foulongne and D. O'Callaghan,200145864Corr, M., D.J. Lee, D.A. Carson and H. Tighe,199618415551560Donnelly, J.J., J.B. Ulmer, J.W. Shiver and M.A. Liu,199715617648Golding, B., D.E. Scott, O. Scharf, L.Y. Huang and Y. Zaitseva et al.,2001Brucella abortus.]]>34348Gregoriadis, G.,199815661670Gurunathan, S., D.M. Klinman and R.A. Seder,200018927974Gurunathan, S., C.Y. Wu, B.L. Freidag and R.A. Seder,200012442447Jiang, X. and C.L. Baldwin,1993Brucella abortus.]]>61124134Jones, S.M. and A.J. Winter,1992Brucella abortus in normal and gamma interferon-activated murine peritoneal macrophages.]]>6030113014Kaufmann, S.H., C.H. Ladel and I.E. Flesch,1995195123132Klinman, D.M., J.M. Sechler, J. Conover, M. Gu and A.S. Rosenberg,199816023882392Ko, J. and G.A. Splitter,2003166578Krishnan, B.R.,200043311Kurar, E. and G.A. Splitter,1997Brucella abortus ribosomal L7/L12 gene elicits immune response.]]>1518511857Lai, W.C. and M. Bennett,199818449484Luo, D., B. Ni, P. Li, W. Shi and S. Zhang et al.,20067427342741Mallick, A.I., H. Singha, S. Khan, T. Anwar and M.A. Ansari et al.,20072578737884Mor, G.,19985511511153Mosmann, T.R. and S. Sad,199617138146Oliveira, S.C. and G.A. Splitter,1995Brucella abortus infection as demonstrated in major histocompatibility complex class I- and class II-deficient mice.]]>2525512557OƱate, A.A., S. Cespedes, A. Cabrera, R. Rivers and A. Gonzalez et al.,2003Brucella abortus induces protective immunity in BALB/c mice.]]>148574861Prud'homme, G.J., B.R. Lawson, Y. Chang and A.N. Theofilopoulos,200122149155Sato, Y., M. Roman, H. Tighe, D. Lee and M. Corr et al1996273352354Smith, L.D. and T.A. Ficht,1990Brucella.]]>17209230Splitter, G., S. Oliveira, M. Carey, C. Miller, J. Ko and J. Covert,199654309319Stevens, M.G., J.W. Pugh and L.B. Tabatabai,1992Brucella abortus infections in mice.]]>6044074409Talwar, G.P., M. Diwan, F. Razvi and R. Malhotra,199912274280Ulmer, J.B., J.J. Donnelly, S.E. Parker, G.H. Rhodes and P.L. Felgner et al.,199325917451749Ulmer, J.B., J.C. Sadoff and M.A. Liu,19968531536