Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2589.2593Tayefi-NasrabadiH. MoghaddamGh. RajabiZ. 1220081122In this study, for clarifying some possible mechanism
of zinc toxicity, the effect of increasing amounts of Zn2+
ion on peroxidase activity was investigated invitro in
serum of cow. The H2O2-mediated oxidation of o-dianisidine
was used to assess the peroxidase activity. Results show that after preincubation
of serum with 0.2-20 mM Zn2+ concentration for 5 min, peroxidase
activity was inhibited compared to the control and decreased rapidly with
increasing metal concentrations. The enzyme was completely inhibited after
5 min preincubation in 30 mM Zn2+. When the preincubation of
serum and Zn2+ was prolonged to 30 and 60 min, the enzymatic
activity decreased more rapidly with increasing metal concentration. Extended
exposure of the enzyme to lower concentrations of the metal brought about
the same effect as shorter exposure to higher metal concentrations.]]>Bexfield, N., J. Archer and M. Herrtage, 2007174414417Bishop, G.M., R. Dringen and S.R. Robinson, 20074212221230Christopher, J.F., 2004101825Dineley, K.E., T.V. Votyakova and I.J. Reynolds, 200385563570Ebisch, I.M, W.L. Van Heerde, C.M. Thomas, N. Van Der Put, W.Y. Wong and R.P. Steegers-Theunissen, 20038011901194Fieldes, M.A. and K.E. Gerhardt, 19981328999Gadallah, M.A.A., 200044451467Gazaryan, I.G., I.P. Krasinskaya, B.S. Kristal and A.M. Brown, 20072822437324380Gupta, R.,20071st Edn.,pp: 422-425pp: 422-425Hershfinkel, M., F. Silverman William and I. Sekler, 200713331336Jeffcott, L.B., 199123331338Keyhani, J., E. Keyhani, S. Zarchipour, H. Tayefi-Nasrabadi and N. Einollahi, 20051722312323Pillai, A. and S. Gupta,20052+.]]>39707712Plumlee, K.H., 20042nd Edn.,Pong, K., Y. Rong, S.R. Doctrow and M. Baudry, 2002950218230Radostits, O.M., C.C. Gay, D.C. Blood, K.W. Hinchcliff, C. Gay and K. Hinchcliff,20009th Edn.,Pages: 1877Pages: 1877Religa, D., D. Strozyk, R.A. Cherny, I. Volitakis and V. Haroutunian et al.,2006676975Richards, K.D., E.J. Schott, Y.K. Sharma, K.R. Davis and R.C. Gardner, 1998116409418Samour, J., 20001st Edn.,Sharpe, RT. and C.T. Livesey, 2005157465469Smith, O.B. and O.O. Akinbamijo,200060-61549560Sousa, S.F., P.A. Fernandes and M.J. Ramos, 200712913781382Suruki, T., K. Nakajima, A. Yamamoto and H. Yamanaka, 199527161164Tayefi-Nasrabadi, H., E. Keyhani and J. Keyhani, 20068811831197Tayefi-Nasrabadi, H., G.H. Moghaddam, Z. Rajabi and G.h. Dehghan, 2008in vitro study.]]>7866869Wong, W.Y., H.M. Merkus, C.M. Thomas, R. Menkveld, G.A. Zielthuis and R.P. Steegers-Theunissen, 200277491498Worthington, C.C., 19881st Edn.,pp: 254-260pp: 254-260Wroblewski, N., W.B. Schill and R. Henkel, 20037915841589Kendall, N.R., S. McMullen, A. Green and R.G. Rodway,200062277283