Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2624.2628Cowpea mild mottle virus Isolated from Soybean in Iran]]>TavassoliM. ShahraeenN. GhorbaniS. 1220081123During 2006-2007 growing seasons, survey were carried to identify a virus disease causing mosaic of soybean in the field in Southern region (Khozestan Province) of Iran. To detect the viral infection, diseased leaf samples showing mild mosaic and leaf malformation were collected from soybean fields in Dezful, located in Khozestan Province. Infected samples were carried to the lab in a proper condition on ice packages. TPIA and DAS-ELISA serological tests were applied to identify the viral agent. To investigate the host-range, several indicator plants were mechanically inoculated under green-house condition. Seed transmission of CPMMV was examined using the seeds obtained from infected plants. The virus isolate was not found to be seed-borne in Clark variety of soybean. Different steps of ultracentrifugation including sucrose density gradient (10-40%) were carried out in order to obtain partial purified virus. On the basis of biological, serological and EM results, CPMMV-Carla virus was identified in the infected soybean samples. This is the first report of CPMMV infection of soybean in Iran.]]>Anno-Nyako, F.O.,1986Cowpea mild mottle virus on soybean by the whitefly Bemisia tabaci in Nigeria.]]>63193194Antignus, Y. and S. Cohen,1987Cowpea mild mottle virus in Israel.]]>110563569Hartman, G.L., J.B. Sinclair and J.C. Rupe, 19994th Edn.,Brunt, A.A. and R.H. Kenten,1973Cowpea mild mottle, a newly recognized virus infecting cowpeas (Vigna unguiculata) in Ghana.]]>746774Brunt, A.A. and S. Philips,1981Lycopersicon esculentum) in Western Nigeria induced by cowpea mild mottle virus.]]>58177180Clark, M.F. and A.N. Adams,197734475483El-Hammady, M., S.E. Albrechtsen, A.M. Abdelmonem, F.M. Abo El-Abbas and W. Gazalla,200412839850Giovanni, P., P. Martelli, M.J. Adams, J. F. Kreuze and V.V. Dolja,20074573100Ghorbani, S.G.M., N. Shahraeen and A. Elahinia,2008Vigna unguiculata) viruses in Guilan province, Iran.]]>12832Ghorbani, S.G.M., N. Shahraeen, A. Elahinia and K. Bananej,2007Phaseolus vulgaris) in Guilan province.]]>2007pp: 11Golnaraghi, A.R., N. Shahraeen, R. Pourrahim, S. Farzadfar and A. Ghasemi,20048810691074Iwaki, M., P. Thongmeearkom, M. Prommin, Y. Honda and T. Hibi,1982Cowpea mild mottle virus on soybean in Thailand.]]>66365368Irma, G., J. Laguna, D. Arneodo, P. Rodriguez and M. Fiorona,2006313942Jeyanandarajah, P. and A.A. Brunt,1993137148156Lister, R.M., 19786813931400Mink, G.I and C.L. Keswani,1987Cowpea mild mottle virus on bean and mung bean in Tanzania.]]>71557557Milne, R.G. and D.E. Lesemann,19841984Tropics, S.A. and P.O. Patancheru,1983Cowpea mild mottle virus by Bemisia tabaci in a nonpersistent manner.]]>67391393Naidu, R.A., S. Gowda, T. Satyanarayana, V. Boyko, A.S. Reddy, W.O. Dawson and D.V.R. Reddy,1998Cowpea mild mottle virus belong to the genus Carlavirus.]]>143769780Nolt, B.L. and R. Rajeshwari,1987Cowpea mild mottle virus isolate from groundnut.]]>402226Pappu, S.S., R. Brand, H.R. Pappu, E.P. Rybicki, K.H. Gough, M.J. Frenkel and C.L. Niblett,199341920Reddy, D.V.R.,199170665678Rodriguez, P.E. and J.D. Arneodo,2004Cowpea mild mottle virus in bean crops in Argentina.]]>33129130