Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2653.2659Hyoscyamus muticus L. using Colchicine Treatment]]>Shahriari-AhmadiF. DehghanE. FarsiM. AziziM. 1220081124In this study the tip of meristem in diploid plants (2n = 2x =28) were treated with a 0.2% colchicine solution and flowcytometry was used to determine the DNA ploidy level of different tissues in individual plants. Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants. We show that, the DNA ploidy of Hyoscyamus muticus, treated with Colchicine can be evaluated using flow cytometry. In addition we also report reliable morphological markers to identify putative tetraploids in this species. Although, tetraploidy was induced using this technique, mixoploid plants were also observed among the Egyptian henbane plants. The results of this study also indicate that colchicine treatment can efficiently be used to improve Hyoscyamus muticus for commercial and pharmaceutical applications of its vegetative organs.]]>Biradar, D.P., A.L. Rayburn and D.G. Bullock,1993Zea mays L.).]]>71417421Chakraborti, S.P., K. Vijayan, B.N. Roy and S.M.H. Qadri,1998Morus alba L.).]]>17799803Cohen, D. and J.L. Yao,1996Zantedeschia cultivars.]]>474349Compton, M.E., D.J. Gray and G.W. Elmstrom,1996in vitro.]]>87165172Dhawan, O.P. and U.C. Lavania,1996878189Dolezel, J., P. Binarova and S. Lucretti,198931113120Galbraith, D.W., K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady,198322010491051Gao, S.L., D.N. Zhu, Z.H. Cai and D.R. Xu,1996477377Gu, X.F., A.F. Yang, H. Meng and J.R. Zhang,2005In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua.]]>24671676Hamill, S.D., M.K. Smith and W.A. Dodd,1992In vitro induction of banana autotetraploids by colchicine treatment of micropropagated diploids.]]>40887896Hassawi, S.D. and G.H. Liang,199131723726Joachimiak, A., A.E, Kula, A. Sliwiska and S.K.A. Sobieszczan,2001Bromus species.]]>43105115Koutoulis, A., A.T., Roy, A. Price, L. Sheriff and G. Legget,2005Humulus lupulus L.).]]>105263268Lavania, U.C.,1986Hyoscyamus muticus L. through induced tetraploidy.]]>73292298Lavania, U.C. and S. Srivastava,1991Hyoscyamus niger L.]]>527377Lavania, U.C., 20053170177Mergen, F. and D.T. Lester,19717314319Pal Bais, H., V.M. Loyola-Vargas, H.E. Flores and J.M. Vivanco,200137730741Panda, D., B.L. Goode, S.C. Feinstein and L. Wilson,1995341111711127Poehlman, J.M.,19873rd Edn.,Roy, A.T., G. Leggett and A. Koutoulis,2001Humulus lupulus L.).]]>20489495Tambong, J.T, V.T, Sapra and S. Garton,1998In vitro induction of tetraploids in colchicine-treated cocoyam plantlets.]]>104191197Thiem, B. and E. Sliwinska,2003Rubus chamaemorus L.) in vitro cultures.]]>164129134Vera, P., C. Lamb and P.W. Doerner,19946717727Wan, Y., J.F. Petolino and J.M. Widholm,198977889892Zehra, M., S. Banerjee, A.A. Naqvi and S. Kumar,1998Hyoscyamus albus, H. muticus and their somatic hybrid.]]>1369399