Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.583.588in vitro]]>RaminM. AlimonA.R. PanandamJ.M. SijamK. JavanmardA. AbdullahN. 42008114The digestion and Volatile Fatty Acid (VFA) production
from rice straw and oil palm fronds by cellulolytic bacteria isolated
from the termite Coptotermes curvignathus were investigated. The
bacteria were Acinetobacter strain Raminalimon, Enterobacter
aerogenes strain Razmin C, Enterobacter cloacae strain Razmin
B, Bacillus cereus strain Razmin A and Chryseobacterium kwangyangense
strain Cb. Acinetobacter strain Raminalimon is an aerobic bacterium,
while the other species are facultative anaerobes. There were significant
differences (p<0.05) among the bacteria for Dry Matter (DM) lost and
acetic acid production from rice straw and Acinetobacter strain
Raminalimon showed the highest activity. The facultative bacteria
C. kwangyangense strain Cb (cfu mL-1 231x10-6,
OD: 0.5), E. cloacae (cfu mL-1 68x10-7, OD:
0.5) and E. aerogenes (cfu mL-1 33x10-7,
OD: 0.5) were used for digestion study with the rumen fluid microflora.
The in vitro gas production technique was applied for the comparative
study and the parameters measured were pH, gas (volume), dry matter lost,
acetic acid, propionic acid and butyric acid concentrations. pH was not
significantly (p<0.05) different among the five treatments. The bacterium
C. kwangyangense strain Cb showed the highest activity (p<0.05)
for DM lost, acetic acid, propionic acid and butyric acid production from
rice straw when compared to the other bacterial activities. There was
no significance (p<0.05) difference between the three bacteria for
the dry matter lost of oil palm fronds but the production of Volatile
Fatty Acids (VFA) was significantly (p<0.05) high in the treatment
which was inoculated with C. kwangyangense strain Cb. The Gen Bank
NCBI/EMBL accession numbers for the bacterial strains are EU332791, EU305608,
EU305609, EU294508 and EU169201.]]>Adams, L. and R. Boopathy, 20059615921598Blummel, M., H. Steingaβ and K. Becker,1997in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages.]]>77911921Borji, M., S. Rahimi, G. Ghorbani, J. Vand Yoosefi and H. Fazaeli,200358249256Brandelli, A. and A. Riffel,200583542Breznak, J.A. and A. Brune,199439453487Brune, A., 1998161621Brune, A. and M. Friedrich, 20003263269Deschamps, A.M., G. Mahoudeau and J.M. Lebeault, 198094551Droge, S., J. Frohlich, R. Radek and H. Konig, 2006Spirochaeta coccoides sp. nov., a novel coccoid spirochete from the hindgut of the termite Neotermes castaneus.]]>72392397Dugas, J.E., L. Zurek, B.J. Paster and B.A. Keddie,2001Chryseobacterium strain from the gut of the American cockroach, Periplaneta americana.]]>175259262Eutick, M.L., R.W. O’Brien and M. Slaytor, 197835823828Getachew, G., E.J. DePeters, P.H. Robinson and J.G. Fadel,2005in vitro rumen gas production technique to evaluate microbial fermentation of ruminant feeds and its impact on fermentation products.]]>123-124547559Ghorbani, G.R., D.P. Morgavi, K.A. Beauchemin and J.A.Z. Leedle,20028019771985Harazono, K., N. Yamashita, N. Shinzato, H. Watanabe, T. Fukatsu and R. Kurane, 2003Coptotermes formosanus. ]]>67889892Jetana, T., N. Abdullah, R.M. Halim, S. Jalaludin and Y.W. Ho,1996ad libitum.]]>1996Khamseekhiew, B., J.B. Liang, A.J. Zainal and C.C. Wong, 2002Arachis pintoi in cattle.]]>24209216Konig, H., 2006Bacillus species in the intestine of termites and other soil invertebrates. ]]>101620627Kuhnigk, T., E.M. Borst, A. Ritter, P. Kampfer, A. Graf, H. Hertel and H. Konig,1994177685Lynch, H.A. and S.A. Martin,2002Saccharomyces cerevisiae culture and Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation.]]>8526032608Mcfarlane, J.E. and I. Alli, 1985115963Odelson, D.A. and J.A. Breznak, 19834516021613Purwadaria, T., P.P. Ketaren, A.P. Sinurat and I. Sutikno,2003Glyptotermes montanus) for poultry feed application. ]]>44047Ramin, M., A.R. Alimon, N. Abdullah, J.M. Panandam and K. Sijam,2008Coptotermes curvignathus (Holmgren) present in the Vicinity of University Putra Malaysia.]]>3288292Rymer, C., J.A. Huntington, B.A. Williams and D.I. Givens, 2005In vitro cumulative gas production techniques: History, methodological considerations and challenges.]]>123-124930SAS.,1997Tokuda, G., L. Nathan and H. Watanabe, 200530372380Varma, A., B. Krishna Kolli, J. Paul, S. Saxena and H. Konig,199415928Yang, W.Z., K.A. Beauchemin, D.D. Vedres, G.R. Ghorbani, D. Colombatto and D.P. Morgavi,2004114179193Schafer, A., R. Konrad, T. Kuhnigk, P. Kampfer, H. Hertel and H. Konig,199680471478