Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.1415.1419In vitro Callus Induction and Plant Regeneration From Withania coagulans: A Valuable Medicinal Plant]]>ValizadehJ. ValizadehM. 1220091221Leaf and internode explants of Withania coagulans Dunal
were used to evaluate the effect of growth regulators on the in vitro callus
induction and plant regeneration. Two independent experiments were performed
to establish callus and plant regeneration. In the first experiment, leaf segments
were cultured on MS basal medium fortified with 2, 4-dichlorophenoxyacetic acid
(2, 4-D, 2-4 mg L-1) with combination of 6-benzyladenine (BA, 0.5-1
mg L-1) or kinetin (Kin, 0.5-1 mg L-1). Callus initiation
was observed best in all media after 14-16 days (100%). Highest callus growth
in terms of dry weight (76±5.34 mg) was observed in MS medium fortified
with 2 mg L-1 2, 4-D and 0.5 mg L-1 Kin. In the second
experiment, callusing also obtained from internode segments cultured on MS medium
with the presence of 2, 4-D (2-4 mg L-1) in association with BA (0.25-0.5
mg L-1). Best callusing rates (42%) and dry weight (86±3.68
mg) occurred in the presence of 4 mg L-1 2, 4-D with combination
of 0.25 mg L-1 BA. Shoot induction was obtained just from callus
induced from internode explants on MS medium containing BA (2 mg L-1)
with IBA (0.5 mg L-1), but the yield of shoot regeneration was unsatisfactory
(18-33%). Regenerated shoots were rooted best (100%) on half strength MS medium
containing IBA (2 mg L-1) and produced 23 roots with an average root
length of 6.5±0.41 cm. Rooted plantlets transferred to soil and sand
mixture (2:1) showed 75% survival when transferred to outdoor. The procedure
reported here in vitro culture of W. coagulans for first time.]]>Kulkarni, A.A., S.R. Thangane and K.V. Krishnamoorthy, 2000W. somnifera. ]]>62203209Atta-ur-Rahman, M. Shabbir, M. Yousaf, S. Qureshi, D. EShahwar, A. Naz and M.I. Choudhary,1999Withania coagulans.]]>5213611364Hemalatha, S., R. kumar and M. Kumar,2008Withania coagulana dunal a review.]]>2351358Murashige, T. and F. Skoog,196215473497Negi, M.S., V. Sabharwal, N. Wilson and M.S. Lakshmikumaranl,2006Withania somnifera genotypes.]]>91464471Rout, G.R., S. Samantaray and P. Das,2000In vitro manipulation and propagation of medicinal plants.]]>1891120Saritha, K.V. and C.V. Naidu,2007In vitro flowering of Withania somnifera Dunal.: An important antitumor medicinal plant.]]>172847851Sharada, M., A. Ahuja, K.A Suri, S.P. Vij, R.K. Khajuria, V. Verma and A. Kumar,2007in vitro cultures of Withania somnifera and its association with differentiation.]]>51161164Siddique, N.A., M.A. Bari, S. Shahnewaz, M.H. Rahman, M.R. Hasan, M.S.I. Khan and M.S. Islam,2004Withania somnifera (L.) dunal (Ashwagandha) from nodal segments derived callus an endangered medicinal plant in Bangladesh.]]>4219223Sivanesan, I. and K. Murugesan,2008Withania somnifera Dunal.]]>7551556Tripathi, L. and J.N. Tripathi,20032243253Rani, G. and I.S. Grover,1999In vitro callus induction and regeneration studies in Withania somnifera.]]>572327