Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.397.400MohitiJ. TalebiF. Afkhami-ArdekaniM. 42009124Present researchers studied the relation between insulin
with free and total leptin in type 2 diabetic patients. Thirty non insulin
dependent diabetic obese patients (age: 50 ± 12 year and BMI>30
kg m-2) and thirty non insulin dependent diabetic non obese
patients (age: 49 ± 25 year and BMI<25 kg m-2) were
studied. Free leptin was purified by Gel filtration Chromatography and
the fractions were collected and then their free leptin was measured by
a high sensitive ELISA Kit. Circulation total leptin and insulin were
measured by ELISA. Circulation free and total leptin were significantly
correlated to insulin (p<0.005). Free leptin concentrations were higher
in women than in men (p<0.001). Ratio of free leptin to total in obese
subjects is more than non-obese subjects (0.27 ± 0.1 vs. 0.03 ±
0.04, p<0.001). Ratio of free to total leptin showed a positive correlations
with insulin (r = 0.58, p<0.001) insulin resistance (r = 31, p<0.015)
and BMI (r = 0.86, p<0.001). The majority of leptin which circulates
in obese individuals was free form. Presumably it is bioactive portion
of hormone and thus obese subjects are resistant to free leptin. These
observations are consistent with the view that free leptin levels in diabetes
patients attributed to changes in serum insulin level and insulin resistant.]]>Baile, C.A., M.A. Della-Fera and R.J. Martin,200020105127Bertin, E., N. Rich, N. Schneider, H. Larbre, C. Marcus, V. Durlach and M. Leutenegger,199824229234Barzilai, N., J. Wang, D. Massilon, P. Vuguin, M. Hawkins and Rossetti,199810031053110Ceddia, R.B., H.A. Koistinen, J.R. Zierath and G. Sweeney,20021611631176Chehab, F.F., J. Qiu, K. Mounzih, A. Ewart-Toland and S. Ogus,200260S39S46Fantuzzi, G. and R. Faggioni,200068437446Feemaandez, A.M., I.K. Kim, S. Yakar and J. Dupont,20011519261934Fischer, S.M., S.M. Hanefeld, C. Haffner, U.S Fusch and C. Kohler et al.,200239105110Kieffer, T.J. and J.F. Habener,2000278E1E14Kieffer, T.J. and J.F. Habener,199920876913Laferrere, B., A. Caixas, S.K. Fried, C. Bashore, J. Kim and F.X. Pi-Sunyer,2002146839845Leclercq-Meyer, V., R.V. Considine, A. Sener and W.J. Malaisse,1996229794798Markus, L., C.F. Ebenbichler, S. Kaser, A Sandhofer, W. Helmut, H. Nehoda, F. Aigner and J.R. Patsch, 200210597601Liu, L., G.B. Karkanias, J.C. Morales, M. Hawkins, N. Barzilai, J. Wang and L. Rossetti, 19982733116031167Muoio, D.M., G.L. Dohm, E.B. Tapscott, R.A. Coleman and Leptin,1999276E913E921Nuamah, M.A., N. Sagawa, S. Yura, H. Mise, H. Itoh, Y. Ogawa, K. Nakao and S. Fujii,200350421428Obici, S., Z. Feng, K. Morgan, D. Stein, G. Karkanias and L. Rossetti,200251271275Petersen, K.F., E.A. Oral and S. Dufour,200210913451350Prigeon, R.L., S.E. Kahn, M. Nicolson, J. Moore, A. Morawiecki, E.J. Boyko and D. Porte,19972014761481Seufert, J., T.J. Kieffer, C.A. Leech, G.G. Holz, W. Moritz, C. Ricordi and J. Habener,199984670676Seufert, J.,200453152158Wallace, T.M., J.C. Levy and D.R. Matthews,20042714871495Zhao, A.Z., K.E. Bornfeldt and J.A. Beavo,1998102869873Mohiti, J., M. Afkhami and A. Babaei,20053121125Brabant, G., H. Nave, B. Mayr, M. Behrend, V. van Harmelen and P. Arner,20028739663970