Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.707.711Tobacco streak virus Isolated from Lettuce]]>AbtahiF.S. MotlaghM. Khodai 92009129Tobacco streak virus (TSV) is an ilarvirus with a worldwide distribution. This virus infects many plants and causes significant yield losses. In this study, 300 samples of lettuce were collected from lettuce fields in Tehran Province. Infected plants show symptoms such as: mosaic, vein clearing, vein necrosis, yellowing and leaf distortion. DAS-ELISA (Double Antibody Sandwich-ELISA) was used with a polyclonal antiserum against TSV. Five isolates (T1, T2, T3, T4 and T5), which are collected, respectively from Mohammad Abad (Karaj), Malek Abad (Karaj), Hashtgerd (Karaj), Tarand Balla (Varamin) and Deh mah sin (Pishva) were inoculated on 29 species of Cucurbitaceae, Amaranthaceae, Solanacea, Compositae, Leguminosae and Chenopodiacea. Chenopodium quinoa 6 days after inoculation showed necrotic local lesions. Gomphrena globosa 10 days after inoculation developed chlorotic local lesions. Systemic symptoms were produced in Datura stramonium. Phaseolus vulgaris cv. Red Kidney 5 days after inoculation developed necrotic local lesions. Nicotiana tabacum 7 days after inoculation showed necrotic and chlorotic local lesions. Nicotiana clevelandii 15 days after inoculation developed leaf distortion and vein necrosis. Lactuca sativa 10-15 days after inoculation developed leaf istortion and mosaic. Reverse Transcription Polymerase Chain Reaction (RT-PCR) was performed using one primer pairs designed by DSMZ. An approximately 710 bp fragment was amplified with a specific primer.]]>Bhat, A.I., R.K. Jain, V. Chaudhary, M. Krishna Reddy, M. Ramiah, S.N. Chattannavar and A. Varma,20021350356Bhat, A.I., R.K. Jain, A. Kumar, M. Ramiah and A. Varma,2002Tobacco streak virus.]]>147651658Bhat, A.I., R.K. Jain and M. Ramiah,2002Tobacco streak virus from sunflower and other crops by reverse transcription polymerase chain reaction.]]>55216218Bhat, A.I., A. Kumar, R.K. Jain, R.S. Chander and M. Ramiah,20019292296Bol, J.F.,1999Alfalfa mosaic virus and ilarviruses: Involvement of coat protein in multiple steps of the replication cycle.]]>8010891102Clark, M.F. and A.N. Adams,197734475483Costa, A.S., 19453510291030Fagbenle, H.H. and R.E. Ford,1970Glycine max.]]>60814820Gracia, O. and J.M. Feldman,197480313323Greber, R.S., M.J. Klose and D.S. Teakle,1991Tobacco streak virus in tobacco and its transmission by Microcephalothrips abdominalis and pollen from Ageratum houstonianum. ]]>75450452Kaiser, W.J., S.D. Wyatt and R.E. Klein,199175258264Kaiser, W.J., S.D. Wyatt and G.R. Pesho,1982Tobacco streak virus in Eastern Washington.]]>7215081512Krishnareddy, M., D. Lakshmi Raman, S. Jalali and D.K. Samuel,2003Tobacco streak virus causing necrosis of cucumber (Cucumis sativus) and gherkin (Cucumis anguria) in India.]]>8712641270McDaniel, L.L., R.N. Raid, C.L. Elliot, J.H. Tsai and R.N. Nagata,199276966971Prasada Rao, R.D.V.J., A.S. Reddy, S.V. Reddy, K. Thirumala-Devi and S. Chander Rao et al.,2003Tobacco streak virus in India and transmission by thrips.]]>142365368Regenmortel, M.H.V., C.M. Fauquet, D.H.L. Bishop, M.A. Mayo, J. Maniloff and C.H. Calisher,199714215051518Sharman, M., J.E. Thomas and D.M. Persley,2008Tobacco streak virus in sunflower (Helianthus annuus), cotton (Gossypium hirsutum), chickpea (Cicer arietinum) and mung bean (Vigna radiata) in Australia.]]>32729