Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2010.46.50Streptococcus sp. From an Anaerobic Shea Cake Digester]]>NitiemaL. W.DianouD. SimporeJ. KarouS.D. SavadogoP. W.TraoreA. S.12010131An anaerobic digester fed with shea cake rich in tannins and phenolic compounds rich-shea cake and previously inoculated with anaerobic sludge from the pit of a slaughterhouse, enabled six months acclimatization of the bacteria to aromatic compounds. Afterwards, digester waste water samples were subject to successive culture on media with 1 g L-1 tannic acid allowing the isolation of a bacterial strain coded AB. Strain AB was facultatively anaerobic, mesophilic, non-motile, non-sporulating, catalase and oxidase negative bacterium, namely strain AB, was isolated from an anaerobic digester fed with shea cake rich in tannins and phenolic compounds, after inoculation with anaerobic sludge from the pit of a slaughterhouse and enrichment on tannic acid. The coccoid cells occurred in pair, short or long chains and stained Gram-positive. Strain AB fermented a wide range of carbohydrates including glucose, fructose, galactose, raffinose, arabinose, sucrose, maltose, lactose, starch and cellulose. Optimum growth occurred with glucose and tannic acid at 37°C and pH 8. The pH, temperature and salt concentration for growth ranged from 5 to 9, 20 to 45°C and 0 to 15 g L-1, respectively. Strain AB converted tannic acid to gallic acid. These features were similar to those of the Streptococcus genus. The determination of tannic acid hydrolysis end products, ability to utilize various organic acids, alcohols and peptides, GC% of the DNA, the sequencing of 16S rRNA gene and DNA-DNA hybridization will permit to confirm this affiliation and to determine the species.]]>Brooker, J.D., L.A.O. Donovan, I. Skene, K. Clarke, I. Blackall and P. Muslera,1994Streptococcus caprinus sp.nov., a tannin-resistant ruminal bacterium from feral goats.]]>18313318Dalsager, P.,1984187274Chamkha, M., K.B. Patel, A. Traore, J.L. Garcia and M. Labat,2002Streptococcus gallolyticus strain that decarboxylates protocatechuic and hydroxycinnamic acids and emendation of the species.]]>5939944Champ, M.M.J.,200288307319Haslam, E.,199659205215He, Q., K. Yao, D. Sun and B. Shi,200718465472Hungate, R.E.,19691969pp: 117-132pp: 117-132Karou, D., H.M. Dicko, J. Simpore and A.S. Traore,20054823828Long, R.M., H.M. Lappin-Scott and J.R. Stevens,200920521531Macy, J.M., J.E. Snellen and R.E. Hungate,19722513181323Maranz, S., Z. Wiesman, J. Bisgard and G. Bianchi,2004Vitellaria paradoxa based on variation in fat composition accross the species distribution range.]]>607176Mondal, K.C., D. Banerjee, M. Jana and B.R. Pati,2001295168171Nelson, K.E., A.N. Pell, P. Schofield and S. Zinder,19956132933298Osawa, R.,1990Streptococcus sp. isolated from faeces of Koalas.]]>56829831Ouattara, A.S., S.A. Traore and J.L. Garcia,1992Anaerovibrio burkinabensis sp.nov., a lactate fermenting bacterium isolated from rice field soils.]]>42390397Rohn, S., H.M. Rawel and J. Kroll,20025035663571Sasaki, E., T. Shimada, R. Osawa, Y. Nishitani, S. Spring and E. Lang,2005Apodemus speciosus, feeding on tannin-rich acorns.]]>28358365Smith, A.H., E. Zoetendal and R.I. Mackie,200550197205Widdel, F. and N. Pfennig,1982Desulfobulbus propionicus gen. nov., sp. nov.]]>131360365Bonkoungou, E.G.,1987Butyrospermum paradoxum), Espece Agroforestiere a Usages Multiples.]]>pp: 69pp: 69