Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2011.212.218WAF1 Protein Expression and Apoptosis in Bilharzial Bladder Cancer]]>GhalebKhaled IsmailAlkaladiAli 32011143The FHIT gene alterations may occur as early consequences for the urinary bladder cancer. The present study aimed to identify and analyze the role of homozygous deletion (HZD) and transcriptional alterations of fragile histidine triad (FHIT) gene in the development and progression of bilharzial bladder cancer in Egyptian patients. The possible associations between FHIT abnormalities and some clinical variables that have prognostic impact in bilharzial bladder cancer patients were determined. In addition, the relations between homozygous deletion (HZD) of FHIT gene, FHIT protein, p21WAF1 expression and apoptosis were demonstrated. We investigated 42 human bladder cancer and 10 normal adjacent tissues as a control group. HZD of the FHIT gene, FHIT protein, p21 protein and apoptosis were assessed by PCR, immunohistochemistry and DNA ladder method, respectively. FHIT HZD deletions have expressed a significant correlation with FHIT protein (p≤0.04), p21WAFI protein expression (p≤0.005) and apoptosis (p≤0.03). On the other side, no correlations were detected between FHIT homozygous deletions and tumor type, tumor grade and gender. Therefore, the FHIT gene deletions could be important in the development and/or progression of urinary bladder cancers and may be used as an independent prognostic indicator for the clinical outcome in patients with these tumors.]]>Ah-See, K., T. Cook, I. Pickford, D. Soutar and A. Balmain,19945416171621Andrea, V., C. Sevignani, E. Giarnieri, N.Z.H. Ishii and R. Cesari et al.,20041076077612Buffa, F.M., J.D. Fenwick and A.E. Nahum,20002712581265Bashir, A.L.,200512152153Capuzzi, D., E. Santoro, W.W. Hauck, A.J. Kovatich and F.E. Rosato et al.,2000882434Campiglio, M., Y. Pekarsky, S. Menard, E. Tagliabue, S. Pilotti and C.M. Croce,19995938663869Wolff, D.J.,2007118177181Eissa, S., M. Welam, M. Shakery and A. Khalifa,199948115119Fadl-Elmula, I., S. Kytola, M.E. Leithy, M. Abdel-Hameed and N. Mandahl et al.,20022225Franceschi, S., R. Talamini, S. Barra, A.E. Baron, E. Bidoli, D. Serraino and C. La Vecchia,19905065026507Gomella, L.G., M.J. Mastrangelo, P.A. McCue, Jr. H.C. Maguire, S.G. Mulholland and E.C. Lattime,200116612911295Herman, J., M. Duda and J. Fischer,199477551554Jemal, A., R.C. Tiwari, T. Murray, A. Ghafoor and A. Samuels et al.,200454829Kuball, J., S.F. Wen, J. Leissner, D. Atkins and P. Meinhardt et al.,200220957965Lux, A., W. Bardenhener, D. Michael, F. Brocker and K. Julicher et al.,19971009095Ohta, M., H. Inoue, M.G. Cotticelli, K. Kastury and R. Baffa et al.,199684587597Pavelic, K., N. Despot, S. Levanat and T. Casl,1990371687692Pavelic, K., S. Krizanac, C. Tamara, M. Hadz and S. Radosevic et al.,20017442453Sarad, L., P. Accornero, S. Tornielli, D. Delia and G. Bunone et al.,19992084898492Tanimoto, K., S. Hayashi, E. Tsuchiya, Y. Tokuchi and Y. Kobayashi et al.,200082838843Virgilio, L., M. Shuster, S. Gollin, M.L. Veronese and M. Ohta,19969397709775Webster, L., G. McKenzie and H. Moriarty,2002133112117Zekri, A.R., A.A. Bahnassy, M. Hafez, A.M. El-Shehaby, G.M. Sherif, H.M. Khaled and N. Zakhary,2005208794Greenlee, R.T., T. Murray, S. Bolden and P.A. Wingo,200050733